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American Chemical Science Journal, ISSN: 2249-0205,Vol.: 3, Issue.: 4 (October-December)


Spectrophotometric Method for the Determination of Cefotaxime Sodiumand Cefoperazone Sodium in Pure and Pharmaceutical Dosage Forms


Rania Adel Sayed1*, Wafaa Sayed Hassan1, Magda Yossif El- Mammli1 and Abdalla Shalaby1

1Department of Analytical Chemistry, Faculty of Pharmacy, Zagazig University, El- Gamah street, Zagazig,44519, Egypt.

Article Information


(1) Sang Hak Lee, Department of Chemistry, Kyungpook National University, Daegu, Korea.


(1) F. Belal, University of Mansoura, Egypt.

(2) Sarah Salah, MSA University, Egypt.

Complete Peer review History: http://www.sciencedomain.org/review-history/1941


Accuratespectrophotometric method was developed for the estimation of cefotaxime sodium (I) andcefoperazone sodium (II) in both pure and pharmaceutical dosage forms. The method is based on the reaction of the amino groups of the cited drugs with ninhydrin reagent producing a colored product which absorbs maximally at 568 nm. Beer's law was obeyed in the concentration range of (10-90) and (60-350) μg.mL-1 for drug (I) and (II) respectively .The correlation coefficient (r2),molar absorptivity (ε), sandell sensitivity, detection (LOD) and quantitation limits (LOQ) for the studied drugs were calculated. The proposed method was successfullyapplied for the determination of certain pharmaceutical dosage forms containing the studied drugs.

Keywords :

Cefotaxime sodium; cefoperazone sodium; spectrophotometric; condensation; ninhydrin.

Full Article - PDF    Page 514-525

DOI : 10.9734/ACSJ/2013/5572

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