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American Chemical Science Journal, ISSN: 2249-0205,Vol.: 3, Issue.: 4 (October-December)

Short Communication

A 100% Water Mobile Phase HPLC-PDA Analysis of Tetracycline Antibiotics


Naoto Furusawa1*

1Graduate School of Human Life Science, Osaka City University, Osaka-558-8585, Japan.

Article Information


(1) Sang Hak Lee, Professor, Department of Chemistry, Kyungpook National University, Daegu, 702-701, Korea.


(1) Anonymous.

(2) Anonymous.

(3) Anonymous.

Complete Peer review History: http://www.sciencedomain.org/review-history/1939


Aims: To develop a reserved-phase HPLC method for detecting oxytetracycline (OTC), tetracycline (TC), and chlortetracycline (CTC) using a 100% water mobile phase.
Study Design: HPLC conditions.
Place and Duration of Study: Author’s Lab., Osaka City University, Japan, between September and November 2012.
Methodology: Chromatographic separations were performed an Inertsil® WP300 C4 (100×4.6 mm, 5 μm) with a water mobile phase and a photodiode-array detector.
Results: The run time was < 5.5 min. The method shows high stability, significant linearity and satisfactory sensitivity. The detection limits were established in the range 0.01 – 0.059 μg/mL.
Conclusion: An organic solvent/reagent-free HPLC method for the simultaneous detection of OTC, TC, and CTC was developed and may be further applied to the quantification in foods.

Keywords :

Oxytetracycline; tetracycline; chlortetracycline; high-performance liquid chromatography; 100% water mobile phase

Full Article - PDF    Page 500-506

DOI : 10.9734/ACSJ/2013/3868

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