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Journal of Scientific Research and Reports, ISSN: 2320-0227,Vol.: 12, Issue.: 3


Detection and Assay of Vitamin B6 (Pyridoxine Hydrochloride) Utilizing Isocratic High Performance Liquid Chromatography


Ronald Bartzatt1*, Purnima Gajmer1, Mai Han Cassandra Nguyen1 and Alexandra My-Hanh Tran1

1University of Nebraska, 6001 Dodge Street, Durham Science Center, Omaha, Nebraska 68182, USA.

Article Information
(1) Amit Balakrishnan, School of Pharmacy, University of Maryland, Baltimore, USA.
(2) Christian Scheckhuber, Cinvestav Monterrey, Apodaca, Mexico.
(1) Pattana Sripalakit, Naresuan University, Phitsanulok, Thailand.
(2) Albert Trokourey, Université Félix Houphouet-Boigny, Ivory Coast.
(3) Feyyaz Onur, Ankara University, Turkey.
(4) Anonymous, University of Pardubice, Czech Republic.
(5) Mindaugas Liaudanskas, Lithuanian University of Health Sciences, Lithuania.
(6) Anonymous, Leiden University, Leiden, The Netherlands.
Complete Peer review History: http://www.sciencedomains.org/review-history/16623


Aims: To demonstrate an analysis for vitamin B6 from commercial aqueous nutritional drinks and solid tablets, utilizing isocratic conditions with high performance liquid chromatography (HPLC) and UV detection at 290 nm.

Study Design: Vitamin B6 in the form of pyridoxine hydrochloride is assayed by HPLC from various samples.

Place and Duration of Study: Department of Chemistry, Durham Science Center, University of Nebraska, Omaha Nebraska from May to August 2016.

Methodology: Utilizing a reversed-phase C-18 column with eluent solvent (19% ethanol, 77% water, 4% acetic acid), the samples were prepared in sample solvent (19% ethanol, 81% distilled water). Detection of vitamin B6 was accomplished at 290 nm. Analysis of samples was done following solubilizing in aqueous conditions having ethanol at 10% to 20% (v/v). Column pressure at 1900 psig, rise time 0.1 with flow rate 1.0 mL/minute. Elution peak for vitamin B6 occurred consistently at 1.6 minutes. Nutritional samples, aqueous samples, and solid pills were prepared in aqueous solvent with various levels of ethanol.

Results: Levels of vitamin B6 detected were as low as 4.4029x10-5 molar to 7.8081x10-4 molar.  Sensitivity for vitamin B6 was highest at 290 nm.  Reverse phase isocratic conditions is shown to be effective for determination of vitamin B6 in aqueous based samples. Standard curves applied are highly linear in range from zero to 7.8081x10-4 molar (y = 112,521,145.5x + 2,818.6), having coefficient of determination (R2 = 0.9948) with very strong positive correlation coefficient (r= 0.9974). Percent recovery of vitamin ranged from 95% to 105%.  Amounts of vitamin present in drinks from same manufacturer were consistent.

Conclusion: Utilizing reversed phase column, isocratic solvent conditions with ethanol in water, and a UV detector set at 290 nm is effective for determination of vitamin B6.  Ethanol-water solvent system is effective. Vitamin B6 was found in various amounts in nutritional drinks tested.

Keywords :

Pyridoxine hydrochloride; vitamin B6; vitamin; HPLC; isocratic.

Full Article - PDF    Page 1-12

DOI : 10.9734/JSRR/2016/28991

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