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British Journal of Medicine and Medical Research, ISSN: 2231-0614,Vol.: 10, Issue.: 8


A New Anionic Bovine Tendon as Scaffold for the Repair of Bone Defects: A Morphological, Histomorphometric and Immunohistochemical Study


Rogerio L. Buchaim1*, Marcelie P. de O. Rosso1, Jesus C. Andreo1, Daniela V. Buchaim2, Roberta Okamoto3, Antonio de C. Rodrigues1, Andre L. Shinohara1, Jose S. Roque4, Domingos D. Roque2, Geraldo M. Rosa Junior5 and Gilberto Goissis6

1Department of Biological Sciences (Anatomy), Bauru School of Dentistry, University of São Paulo,  Al. Dr. Octávio Pinheiro Brisola 9-75, 17012-901, Bauru, SP, Brazil.

2Medical School, Discipline of Human Morphophysiology, University of Marilia (UNIMAR), Marilia, São Paulo, Brazil, R. Hygino Muzy Filho, 17525-902, Marília, SP, Brazil.

3Department of Basic Sciences (Anatomy), Araçatuba School of Dentistry, São Paulo State University (UNESP), R. José Bonifácio 1193, 16015-050, Araçatuba, SP, Brazil.

4Anatomy, Northern Paraná State University (UENP), Av. Manoel Ribas 215, 86400-970, Jacarezinho, PR, Brazil.

5Department of Health Sciences, University of Sacred Heart, R. Irmã Arminda 10-50, 17011-160, Bauru, SP, Brazil.

6Faculty of Medicine of São José do Rio Preto (FAMERP), Av. Brigadeiro Faria Lima 5416, 15090-000, São José do Rio Preto, SP, Brazil.

Article Information


(1) Masahiro Hasegawa, Department of Orthopaedic Surgery, Mie University Graduate School of Medicine, 2-174 Edobashi, Tsu City, Mie, 514-8507, Japan.


(1) Anonymous, University of Rome “Tor Vergata”,  Italy.

(2) Anonymous, The Hong Kong University of Science and Technology, Hong Kong, China.

(3) Anonymous, Universidade do Vale do Paraíba, Brasil.

Complete Peer review History: http://sciencedomain.org/review-history/10678


Aim: The process of bone repair is of evident importance in both the clinical and functional spheres. For this reason, the field of bioengineering has taken it as an object of study, seeking to perfect the implantation of materials that allow for adequate bone neoformation. This study investigated the process of bone repair after anionic bovine tendon grafting in rat tibias by conducting a morphological, histomorphometric and immunohistochemical study.

Methodology: The experimental model consisted of 36 rats randomly divided into two groups: a control group (CG, n=18), in which a surgical cavity in the tibia was filled with blood clots; and an experimental group (EG, n=18), in which a surgical cavity in the tibia was filled with an anionic bovine tendon graft. In the experimental group, the major axis of the collagen fiber bundle was placed perpendicularly to the long axis of the tibia. Microscopic, morphometric and immunohistochemical evaluations were conducted at 7, 15, and 30 days postoperative.

Results: The analyzes showed an increase in bone neoformation in the experimental group during the assessed periods. There was a significant difference between day 7 and day 30 and evident vascular proliferation was detected by the immunohistochemical analysis.

Conclusion: It can be concluded that the anionic bovine tendon collagen proved to be an adequate and biocompatible material for bone regeneration, with osteogenic capabilities that allow it to be used as a scaffold for bone repair.

Keywords :

Bone regeneration; bone transplantation; collagen; immunohistochemistry; tendons; tissue engineering; tissue scaffolds.

Full Article - PDF    Page 1-11

DOI : 10.9734/BJMMR/2015/19678

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