Dr. Prem K. Ramasamy
All India Institute of Medical Sciences, New Delhi, India, Ph.D., Biophysics. Advisor: Professor Punit Kaur. Dissertation title: Structure-based rational drug design: structural studies of phospholipase A2 and its complexes with designed inhibitors. University of Madras, Chennai, India: M.S., Biophysics, Advisor: Professor Giri Rajan. Bharathiar University, Coimbatore, India: B.S., Physics. Technical Skills: Molecular biology: polymerase chain reaction; cloning; site-directed mutagenesis. Protein expression: HEK293; Escherichia coli. Protein purification: FPLC; soluble and membrane proteins using immuno-affinity, immobilized metal affinity (IMAC), size-exclusion (SEC), hydrophobic and ionic chromatography; SDS-PAGE; dialysis; ultrafiltration; western blotting. Analytical techniques: UV-visible spectroscopy; radioactivity based assays; isothermal titration calorimetry (ITC); analytical ultracentrifugation (AUC); gas chromatography–mass spectrometry (GC-MS); high performance liquid chromatography (HPLC); fluorescence spectroscopy.