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Dr. Héctor Manuel Mora Montes

Héctor Manuel Mora Montes   (42 years old)  studied the University degree in Clinical    Chemistry (1993-1997) in Universidad Veracruzana,  where  we  received the award as best undergraduate student, with a    final  average  mark  9.8/10.  Soon after finishing his basic formation, we worked as  Clinical  analyst (1997-2000) in Centro de Especialidades  Médicas  del  Estado  de Veracruz   “Doctor   Rafael   Lucio”.   Veracruz, During  this  post,  he  obtained  valuable  experMieénxceicoa.s  clinical  microbiologist,  carrying  out experiments in the typing of fungi, bacteria and mycobacteria; and developed a system for the differentiation  of  Candida  species,  based  on  in-house  zymograms  for  carbohydrate assimilation, and colony morphology in corn meal agar. In the immunology department, he received training in Western blotting and ELISA for the detection of virus, hormones, bacteria and cancer markers. In the haematology area, we developed skills to identify different classes of white and red blood cells associated with diverse diseases, which was reinforced by a Diplomat in Haematology that finished in 2000. Then, he moved to basic science and started a MSc program  in  Genetics  and  Molecular  Biology  (2000-2002)  in  Departamento  de  Genética  y Biología Molecular del Centro de Investigación y Estudios Avanzados del I.P.N., México, D.F. His  work  was  focused  in  the  biochemical  and  molecular  characterization  of  C. albicans mannosidases,  gaining  experience  in  the  purification  and  biochemical  characterization  of soluble  proteins,  and  becoming  familiar  with  standard  recombinant  DNA technology,  and nucleic acid analysis. He continued with a PhD program in Experimental Biology (2002-2006) in Universidad de Guanajuato, Guanajuato, México, with an experimental work focused in the same topic, C. albicans mannosidases. During this period  he  complemented his  molecular biology and biochemical skills with new techniques, including biochemical characterization of membrane-bound proteins, purification and analysis of carbohydrates using high-performance anion-exchange  chromatography,  generation  of  polyclonal  antibodies,  intracellular  protein localization   using   ultracentrifugation   techniques,   fungal  gene   isolation   and   disruption, characterization  of  mutant  phenotypes  with  defects  in  the  glycosylation  pathways,  and heterologous expression of proteins.  It  is noteworthy  to  mention  that his experience  in biochemistry was translated in the development of a new and innovative method for in situ determination of glycosyl hydrolase activities. He received the award as best PhD student and the “Summa Cum Laude” distinction from Universidad de Guanajuato. During the PhD studies, he has a research-training visit  at the Aberdeen Fungal Group, University of Aberdeen, Aberdeen, U.K., under supervision of Prof. Neil A. R. Gow (2005). In this period, he learnt to generate C. albicans null mutants using the mini-ura-blaster system, and to test the N- linked  and  O-linked  mannosylation  status  using  in  situ  zymograms  and  TLC  assays, respectively. His performance and experience where noticed by Prof. Gow whom offered him a post as postdoctoral research fellow, which was accepted and lasted until 2009. During this post, he further enhanced his experience through the assimilation of relevant methods to study the infectious disease immunology. To achieve this goal, he performed a three-month research training in Radboud University Nijmegen Medical Centre (Nijmegen, The Netherlands, 2007) under supervision of Prof. Mihai G. Netea, which allowed him to learn the isolation and manipulation of several human immune cells (peripheral blood mononuclear cells, macrophages and  dendritic  cells).

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