International Blood Research & Reviews, ISSN: 2321-7219,Vol.: 5, Issue.: 2
Detection of Prethrombin 1 in Human Blood Plasma
Dar’ya Korolova1, Volodymyr Chernyshenko1*, Tetiana Platonova1, Tamara Chernyshenko1 and Eduard Lugovskoy1 1Department of Protein Structure and Function, Palladin Institute of Biochemistry of NAS of Ukraine, 9 Leontovych Street, Kyiv, 01601, Ukraine.
Dar’ya Korolova1, Volodymyr Chernyshenko1*, Tetiana Platonova1, Tamara Chernyshenko1 and Eduard Lugovskoy1
1Department of Protein Structure and Function, Palladin Institute of Biochemistry of NAS of Ukraine, 9 Leontovych Street, Kyiv, 01601, Ukraine.
(1) Armel Hervé Nwabo Kamdje, University of Ngaoundere-Cameroon, Ngaoundere, Cameroon.
(1) Arthur Chu, Wayne State University, USA.
(2) Liana Maria Torres de Araujo Azi, Federal University of Bahia, Brazil.
(3) Robson Q. Monteiro, Federal University of Rio de Janeiro, Brazil.
Complete Peer review History: http://sciencedomain.org/review-history/13761
Background: Prethrombin 1 is one of prothrombin derivatives which appears in the presence of thrombin in vitro. However, existence of prethrombin 1 in vivo remained questionable. The aim of present work was to detect of prethrombin 1 in vivo at abdominal aortic aneurysm, hip replacement after fracture and stroke.
Methodology: Blood plasma samples of patients with abdominal aortic aneurysm, hip replacement after fracture, stroke, and patients which were treated with warfarine were collected. Detection of prethrombin 1 was completed using three independent approaches: combination of prothrombin index and ecamulin (ecarin) index; the APTT-test modified by addition of exogenous prothrombin; Western-blotting of blood plasma using polyclonal antibody to prothrombin.
Results: Prethrombin 1 presence in patients’ blood plasma at studied pathologies was proven. Inactive prothrombin (descarboxy-prothrombin), but not prethrombin 1 was found in blood plasma of patients which were treated with warfarine. Developed approach can be used for testing the prethrombin 1 in clinical diagnostics.
Conclusion: Prethrombin 1 at different pathologies, accompanied by coagulation disorders was detected in patients’ blood plasma directly.
Prethrombin 1; prothrombin; echis multisquamatis, hip-replacement therapy; blood coagulation.
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DOI : 10.9734/IBRR/2016/24683Review History Comments