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Journal of Advances in Biology & Biotechnology, 2394-1081,Vol.: 5, Issue.: 1

Short Research Article

Antioxidant, Anticancer and Antimicrobial, Effects of Rubia cordifolia Aqueous Root Extract

 

R. Barlow1, D. Barnes1, A. Campbell1, P. Singh Nigam1 and R. Owusu-Apenten1*

1School of Biomedical Sciences, University of Ulster, Coleraine, BT52 1SA, UK.

Article Information
Editor(s):
(1) Anil Kumar, Professor & Head (Chair), School of Biotechnology, Devi Ahilya University, Madhya Pradesh, India.
Reviewers:
(1) Ayona Jayadev, All Saints’ College, Kerala, India.
(2) Sahar Mohamed Kamal Shams El Dine, Ain Shams University, Cairo, Egypt.
(3) Ahmed Hegazi, National Research Centre, Egypt.
Complete Peer review History: http://sciencedomain.org/review-history/12260

Abstracts

Aims: To evaluate the total antioxidant capacity (TAC) of Rubia cordifolia root extracts, to test anticancer activity against MDA-MB-231 breast cancer cell lines, and to evaluate antimicrobial activity of the same extract versus six Gram-positive and negative bacteria.

Study Design:  In vitro.

Place of Study and Duration: School of Biomedical Sciences, Ulster University, July 2014-Sept 2015.

Methodology: TAC was tested using ABTS, DPPH, FRAP and Folin assays and values were expressed as mg-gallic acid equivalents per 100 g (GAE/100 g) of sample. Anticancer properties were examined against MDA-MB-231 breast cancer cell lines using Sulforhodamine B assay. Antimicrobial activity was examined using a disk diffusion assay with three Gram-positive (Staphylococcus epidermidis, Staphylococcus aureus and Bacillus cereus) and three Gram-negative (Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi) bacteria.

Results: TAC of dry extracts of Rubia cordifolia ranged from 523±43 to 4513±208 (mg GAE mg/100 g) depending on the method of analysis, ABTS> FRAP> Folin > DPPH methods. R. cordifolia dry extract showed cytotoxicity against MDA-MB-231 with IC50 = 44 µg/ml or 5.1µM GAE. No antimicrobial activity was observed against the three Gram-positive, or three Gram-negative bacterial species using the water extract or R. cordifolia.

Conclusion: R. cordifolia aqueous extract possess high total antioxidant capacity but values depend on the method of analysis. R. cordifolia extract inhibits MDA-MB-231 breast cancer cells proliferation but nil anti-bacterial activity was observed for three Gram-positive and three Gram-negative bacterial strains tested.

Keywords :

Manjistha; Rubia cordifolia; antioxidant; antimicrobial; MDA-MB-231; breast cancer; ABTS; DPPH; FRAP.

Full Article - PDF    Page 1-8

DOI : 10.9734/JABB/2016/22625

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