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British Microbiology Research Journal, ISSN: 2231-0886,Vol.: 8, Issue.: 5


Activity Guided Fractionation with Antimicrobial Evaluation of Pergularia tomentosa L. (Asclepiadacea) Whole Plant


Rufai Yakubu1*, Fatima Musa1, Aminu Lukman1 and Fatima Sheikh1
1Department of Chemistry, Federal College of Education Okene, P.M.B 1026, Kogi State, Nigeria.

Article Information
(1) Lingan Rajendran, Tamil Nadu Agricultural University, India.
(2) Laleh Naraghi, Plant Disease Research Department, Iranian Research Institute of Plant Protection, Tehran, Iran.
(3) Joao Lucio Azevedo, University of São Paulo, Department of Genetics, Brazil.
(1) Cristiane Bezerra da Silva, Department of Pharmacy, Universidade Federal do Paraná, Brazil.
(2) Anonymous, Iuliu Hațieganu University of Medicine and Pharmacy, Romania.
Complete Peer review History: http://www.sciencedomain.org/review-history/9387


This research work aimed to present the activity guided fractionation with antimicrobial evaluation from both crude extract and various fractions obtained from Pergularia tomentosa L. whole plant. The P. tomentosa L. whole plant was extracted with 95% aqueous ethanol; fractionated into acidic, basic, polar and nonpolar fractions. All fractions with the crude extract were screened for both antimicrobial and minimum inhibitory concentration (MIC) potentialities. For crude extract, all concentrations (1.5, 0.75, 0.35, & 0.168 mg/ml) indicated marginal antibacterial activity with range of 17, 20, 14 mm zone of inhibition for S. aureus, E. coli and C. albicans. While, both basic fraction showed highest activity against E. coli and C. albicans at 15 mm & 15 mm; along with acidic and methanolic fraction haven large spectrum against S. aureus, E. coli and C. albicans at 13, 12, 12 mm. Moreover hexane did not showed antimicrobial activity for both S. aureus and C. albicans except for E. coli which showed activity at 12 mm. The study clearly indicated that basic fraction showed highest antimicrobial activity for selected micro-organisms with lower minimum inhibitory concentration which ranges from 18.75 µg/ml to 150 µg/ml. Followed by wider spectrum of antimicrobial activity for acidic and methanolic fraction against all tested organisms with minimum inhibitory concentration from 75 µg/ml to 150 µg/ml; while 300 µg/ml (MIC) stand for hexane fraction. Thus, P. tomentosa L. particularly, the basic fraction (alkaloid) and, both acidic and neutral fractions will be centered areas for further scientific research findings in isolating an active antimicrobial component therein.

Keywords :

P. tomentosa L.; phytochemical analysis; bioassay guided fractionation protocol; Staphylococcus aureus and Escherichia coli and Candida albicans.

Full Article - PDF    Page 567-576

DOI : 10.9734/BMRJ/2015/17027

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