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European Journal of Medicinal Plants, ISSN: 2231-0894,Vol.: 8, Issue.: 3

Original-research-article

In vitro Anti-inflammatory Evaluation of African Nutmeg (Monodora myristica) Seeds

 

K. F. Akinwunmi1* and O. O. Oyedapo1

1Department of Biochemistry, Faculty of Science, Obafemi Awolowo University, Ile-Ife, Nigeria.

Article Information
Editor(s):
(1) Marcello Iriti, Faculty of Plant Biology and Pathology, Department of Agricultural and Environmental Sciences, Milan State University, Italy.
Reviewers:
(1) Anonymous, University Malaysia Pahang, Malaysia.
(2) Muhammad Aslam, Department of Basic Medical Sciences, Faculty of Pharmacy, Ziauddin University, Karachi, Pakistan.
(3) Anonymous, Medical University, Lublin, Poland.
Complete Peer review History: http://www.sciencedomain.org/review-history/9051

Abstracts

Aim: To determine the anti-inflammatory potential of flavonoid rich fraction of Monodora myristica seeds.
Study Design: In vitro evaluation of anti-inflammatory assays: Membrane stability, Inhibition of denaturation of albumin and lipoxygenase inhibition.
Place and Duration: Department of Biochemistry, Obafemi Awolowo University, Ile-Ife, Nigeria (May–November, 2014).
Methodology: Flavonoid rich fraction of the seeds of M. myristica was obtained from the ethanol extract of M. myristica seeds by solvent partitioning. Standard methods were employed in the anti-inflammatory assays (Membrane stability, Inhibition of denaturation of albumin and lipoxygenase inhibition).
Results: Flavonoid rich fraction of M. myristica exhibited significant in vitro anti-inflammatory potentials by stabilizing red blood cell membrane exposed to hypotonic and heat induced lyses with maximum percentage stability of 88±0.45% in a biphasic mode of response that is comparable with Ibuprofen a standard anti-inflammatory drug. It also inhibits heat induced albumin denaturation with maximum inhibition of 75.38±0.56% in a concentration dependent manner that is comparable with aspirin. Flavonoid rich fraction of M. myristica showed an anti-lipoxygenase activity range from 19±1.28% to 71±2.13% which is comparable to that produced by indomethacin.
Conclusion: The result obtained in the present investigation indicates that flavonoid rich fraction of M. myristica could be a potential source of anti-inflammatory agent. This substantiates the role of M. myristica seeds as a natural source of anti-inflammatory agent.

Keywords :

Monodora myristica; anti-inflammatory; denaturation; membrane stability; red blood cells (RBC).

Full Article - PDF    Page 167-174

DOI : 10.9734/EJMP/2015/17853

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