European Journal of Medicinal Plants, ISSN: 2231-0894,Vol.: 8, Issue.: 3
In vitro Anti-inflammatory Evaluation of African Nutmeg (Monodora myristica) Seeds
K. F. Akinwunmi1* and O. O. Oyedapo1 1Department of Biochemistry, Faculty of Science, Obafemi Awolowo University, Ile-Ife, Nigeria.
K. F. Akinwunmi1* and O. O. Oyedapo1
1Department of Biochemistry, Faculty of Science, Obafemi Awolowo University, Ile-Ife, Nigeria.
(1) Marcello Iriti, Faculty of Plant Biology and Pathology, Department of Agricultural and Environmental Sciences, Milan State University, Italy.
(1) Anonymous, University Malaysia Pahang, Malaysia.
(2) Muhammad Aslam, Department of Basic Medical Sciences, Faculty of Pharmacy, Ziauddin University, Karachi, Pakistan.
(3) Anonymous, Medical University, Lublin, Poland.
Complete Peer review History: http://www.sciencedomain.org/review-history/9051
Aim: To determine the anti-inflammatory potential of flavonoid rich fraction of Monodora myristica seeds.
Study Design: In vitro evaluation of anti-inflammatory assays: Membrane stability, Inhibition of denaturation of albumin and lipoxygenase inhibition.
Place and Duration: Department of Biochemistry, Obafemi Awolowo University, Ile-Ife, Nigeria (May–November, 2014).
Methodology: Flavonoid rich fraction of the seeds of M. myristica was obtained from the ethanol extract of M. myristica seeds by solvent partitioning. Standard methods were employed in the anti-inflammatory assays (Membrane stability, Inhibition of denaturation of albumin and lipoxygenase inhibition).
Results: Flavonoid rich fraction of M. myristica exhibited significant in vitro anti-inflammatory potentials by stabilizing red blood cell membrane exposed to hypotonic and heat induced lyses with maximum percentage stability of 88±0.45% in a biphasic mode of response that is comparable with Ibuprofen a standard anti-inflammatory drug. It also inhibits heat induced albumin denaturation with maximum inhibition of 75.38±0.56% in a concentration dependent manner that is comparable with aspirin. Flavonoid rich fraction of M. myristica showed an anti-lipoxygenase activity range from 19±1.28% to 71±2.13% which is comparable to that produced by indomethacin.
Conclusion: The result obtained in the present investigation indicates that flavonoid rich fraction of M. myristica could be a potential source of anti-inflammatory agent. This substantiates the role of M. myristica seeds as a natural source of anti-inflammatory agent.
Monodora myristica; anti-inflammatory; denaturation; membrane stability; red blood cells (RBC).
Full Article - PDF Page 167-174
DOI : 10.9734/EJMP/2015/17853Review History Comments