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European Journal of Medicinal Plants, ISSN: 2231-0894,Vol.: 7, Issue.: 1


HPLC Profiling and Antioxidant Properties of the Ethanol Extract of Hibiscus tiliaceus Leaf Available in Bangladesh


Hemayet Hossain1*, Proity Nayeeb Akbar1, Shaikh Emdadur Rahman2, Sabina Yeasmin2, Tanzir Ahmed Khan3, Md. Mahfuzur Rahman3 and Ismet Ara Jahan1

1BCSIR Laboratories, Bangladesh Council of Scientific and Industrial Research, Dhaka, Dr. Qudrat-E-Khuda Road, Dhaka-1205, Bangladesh.
2Pharmacy Discipline, Life Science School, Khulna University, Khulna-9208, Bangladesh.
3Institute of Food Science and Technology, Bangladesh Council of Scientific and Industrial Research, Dhaka, Dr. Qudrat-E-Khuda Road, Dhaka-1205, Bangladesh.

Article Information
(1) Thomas Efferth, Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, Johannes Gutenberg University, Germany.
(2) Marcello Iriti, Department of Agricultural and Environmental Sciences, Milan State University, Italy.
(1) Asaduzzaman Khan, The Research Center for Preclinical Medicine, Luzhou Medical College, Sichuan, China.
(2) Anonymous, Taiwan.
(3) Slobodan Jankovic, Pharmacology and toxicology department, Faculty of Med. Sciences, University of Kragujevac, Serbia.
(4) Nicola Caporaso, Department of Agriculture, University of Naples Federico II, Italy.
(5) Anonymous, Egypt.
Complete Peer review History: http://www.sciencedomain.org/review-history/8065


The aim of this research was to investigate the antioxidant activity and HPLC fingerprinting profiles of the ethanolic leaf extract of Hibiscus tiliaceus growing in Bangladesh. Catechin, rutin hydrate, ellagic acid and quercetin contents were quantified in the sample by HPLC-DAD (99.00±1.88, 79.20±1.59, 59.40±1.36 and 69.30±1.47 mg/100 g of dry extract), respectively. The antioxidant potential by 2,2-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid (ABTS) radical scavenging activity, reducing power, total antioxidant capacity and total phenolic and flavonoid content were determined. In all the methods, the extract exhibited potent antioxidant activity. The IC50 value of the extract on ABTS radical was found to be 6.25±0.15 μg/ml, and at 250 µg/ml, maximum absorbance of reducing power was obtained 0.6475±0.021. The total antioxidant capacity, total phenolic and flavonoid contents were found in considerable amount (595.2±3.61 mg of ascorbic acid/g, 298.07±2.01 mg/g of gallic acid, and 13.69±0.06 mg/g of quercetin equivalent), respectively. Substantial amounts of phenols and flavonoids were noticed and thus, justify the free radical scavenging and antioxidant characteristics of the extract.

Keywords :

Hibiscus tiliaceus, antioxidant, HPLC, rutin hydrate, ellagic acid, quercetin, ABTS, reducing power.

Full Article - PDF    Page 7-15

DOI : 10.9734/EJMP/2015/14720

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