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American Journal of Experimental Agriculture, ISSN: 2231-0606,Vol.: 7, Issue.: 1

Original-research-article

Plant Regeneration from Shoot Tips-derived Callus of Ginger (Zingiber officinale Rosc.)

 

Diaa A. Ibrahim1*, Gharbia H. Danial1, Vian M. Mosa1 and Belan M. Khalil1

1Department of Scientific Research Center, Faculty of Science, University of Duhok, Iraq.

Article Information
Editor(s):
(1) Lixiang Cao, Department of Biotechnology, Sun Yat-sen University, P. R. China.
(2) Anonymous.
Reviewers:
(1) Arvinder Kapur, University of Wisconsin, USA.
(2) Anonymous, India.
(3) Anonymous, Malaysia.
(4) Anonymous, Malaysia.
Complete Peer review History: http://www.sciencedomain.org/review-history/7908

Abstracts

A protocol was described to regenerate plants from callus culture of ginger (Zingiber officinale Rosc.). Callus cultures were induced from ginger shoot tips inverted cultured on semi-solid MS medium supplemented with 2, 4-D (0.5-1.0 mg L-1) and BA (0.5- 1.0 mg L-1). Maximum callus induction was obtained on MS medium supplemented with BA (0.5 mg L-1) either alone or with 2,4-D (0.5 – 1.0 mg L-1), while the highest callus weight was on MS medium supplemented with BA (0.5 mg L-1) and 2,4-D (0.5 mg L-1). Induced callus was tested for regeneration on MS medium supplemented with different concentrations of 2,4-D and BA and the best regeneration was observed on a medium containing 2.5 mg L-1 BA. The regenerated plants were rooted and successfully established in the field after few days of acclimatization.

Keywords :

Ginger; callus induction; plant regeneration; shoot tips.

Full Article - PDF    Page 55-61

DOI : 10.9734/AJEA/2015/13980

Review History    Comments

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