American Journal of Experimental Agriculture, ISSN: 2231-0606,Vol.: 7, Issue.: 1
Plant Regeneration from Shoot Tips-derived Callus of Ginger (Zingiber officinale Rosc.)
Diaa A. Ibrahim1*, Gharbia H. Danial1, Vian M. Mosa1 and Belan M. Khalil1 1Department of Scientific Research Center, Faculty of Science, University of Duhok, Iraq.
Diaa A. Ibrahim1*, Gharbia H. Danial1, Vian M. Mosa1 and Belan M. Khalil1
1Department of Scientific Research Center, Faculty of Science, University of Duhok, Iraq.
(1) Lixiang Cao, Department of Biotechnology, Sun Yat-sen University, P. R. China.
(1) Arvinder Kapur, University of Wisconsin, USA.
(2) Anonymous, India.
(3) Anonymous, Malaysia.
(4) Anonymous, Malaysia.
Complete Peer review History: http://www.sciencedomain.org/review-history/7908
A protocol was described to regenerate plants from callus culture of ginger (Zingiber officinale Rosc.). Callus cultures were induced from ginger shoot tips inverted cultured on semi-solid MS medium supplemented with 2, 4-D (0.5-1.0 mg L-1) and BA (0.5- 1.0 mg L-1). Maximum callus induction was obtained on MS medium supplemented with BA (0.5 mg L-1) either alone or with 2,4-D (0.5 – 1.0 mg L-1), while the highest callus weight was on MS medium supplemented with BA (0.5 mg L-1) and 2,4-D (0.5 mg L-1). Induced callus was tested for regeneration on MS medium supplemented with different concentrations of 2,4-D and BA and the best regeneration was observed on a medium containing 2.5 mg L-1 BA. The regenerated plants were rooted and successfully established in the field after few days of acclimatization.
Ginger; callus induction; plant regeneration; shoot tips.
Full Article - PDF Page 55-61
DOI : 10.9734/AJEA/2015/13980Review History Comments