Quick Menu

Upcoming Journals

International Journal of TROPICAL DISEASE & Health

International Journal of TROPICAL DISEASE & Health, ISSN: 2278-1005,Vol.: 2, Issue.: 3 (July-September)

 

Short Communication

 

Effect of Storage Temperature and Sample Volume on Brucella melitensis Isolation from Goat Milk

 

 

Jennifer A. Zambriski1*, Ryan C. Maves2, Daryl V. Nydam3, Viterbo Ayvar4, David Cepeda2, Rosa Castillo2, Andre Díaz-Pino4, Mayuko Saito5 and Robert H. Gilman6

1Cornell University, Department of Animal Science, Ithaca, NY 14853, U.S.A.
2Naval Medical Research Unit SIX, Avenida Venezuela Cuadra 36 s/n, Callao 02, Peru.
3Cornell University, Veterinary Medical Center, Room C2 562, Ithaca, NY 14853, U.S.A.
4Centro de Salud Global UPCH, Tumbes, Panamericana Norte Km 1275, Puerto el Cura – Tumbes, Peru.
5AsociaciÏŒn Benéfica PRISMA, Carlos Gonzales 251, Urb. Maranga, San Miguel, Lima, 32, Peru.
6Johns Hopkins University, Bloomberg School of Public Health, 615 N Wolfe St. Rm W5515, Baltimore, MD 21205, U.S.A.

 

Abstracts

 

Aim: To determine the impact of storage temperature and sample volume on milk culture success under a simulated field setting.
Study Design: Prospective cohort study.
Place and Duration of Study: Centro de Salud Global UPCH, Tumbes, Peru and Naval Medical Research Unit SIX, Lima, Peru. April, May and June 2010.
Methodology: We aseptically collected milk from unvaccinated goats, and then experimentally inoculated the pooled milk sample with B. melintensis in order to compare the effect of two different sample volumes (2ml and 5ml) and two different storage temperatures (4ºC and -20ºC) on culture success.
Results: We achieved higher culture success in refrigerated (4ºC) versus frozen (-20ºC) samples (OR 4, 95% CI 1.7, 9.6) and with 5-ml versus 2-ml aliquots (OR 9, 95% CI 3.3, 26.6).
Conclusion: In resource-poor field settings where cold-chain and transportation are unreliable, use of ice for sample storage and transport of goat milk is an acceptable method for the purpose of culturing B. melintensis.

 

Keywords :

Brucellosis; goat; peru; milk; culture; refrigeration; freeze; storage.

 

Full Article - PDF    Page 207-213    Article Metrics

 

DOI : 10.9734/IJTDH/2012/1738

Review History    Comments

Search this site

Advanced Search

Announcement & News

SCOPUS selected Annual Research & Review in Biology (ARRB)

We are delighted to inform that famous indexing organization SCOPUS (from Elsevier) selected  A...

Index Copernicus Evaluation Result Released

We are delighted to inform that Index Copernicus (a leading indexing organization from Pol...

Journal Repository (JR): Permanent Digital Archiving of SDI journals

SDI is happy to announce that all our journals are now permanently archived in Journal Repository (J...

Index Copernicus Value of British Journal of Medicine and Medical Research

  We are delighted to inform that Index Copernicus (a leading indexing organization ...

SDI journal got 35th ranking in Publons

We are delighted to announce (as of 04/01/2016) that British Journal of Medicine and Medical Re...

Growth of SDI and world publication market

As of 2014, total 25,064 journals are competing in World market of journal publication. In 2011, tot...

Publication and site statistics (up to Sept, 2015)

  Number of submission: 22181 (Total up to Sept, 2015) Number of published paper: 10949&n...

Index Medicus selected 15 SDI journals

We are delighted to inform that Index Medicus (IMSEAR) has selected following 15 SDI journals for in...

SCIENCEDOMAIN international received 5844 submissions in 2013

SCIENCEDOMAIN international is pleased to announce that it has received 5844 submissions in 2013, ac...

Science (IF: 31) report confirmed the high standard of SDI journal

As per a recent report (Link) of Science journal (present Impact factor 31), one of our journal (Bri...



SCIENCEDOMAIN Awards

Browser Compatibility : Mozila firefox, Google Crome and IE 7 & above. Creative Commons License Terms & Condition   |   Privacy Policy   |   Join Us   |   Help   |   Contact Us
© Copyright 2010-2017, SCIENCEDOMAIN international. All rights reserved.