British Journal of Medicine and Medical Research, ISSN: 2231-0614,Vol.: 5, Issue.: 10
Molecular Identification of Mycobacterium bovis from Post-mortem Inspected Cattle at the Abattoir and Slaughter Houses in Bauchi State, Nigeria
A. S. Sa’idu1*, E. C. Okolocha1, A. A. Dzikwi1, J. K. P. Kwaga1, A. Usman2, A. A. Gamawa3, U. B. Abubakar4 and S. A. Maigari5 1Department of Veterinary Public Health and Preventive Medicine, Ahmadu Bello University, P.M.B. 1013, Zaria, Kaduna State, Nigeria.
2Tuberculosis Laboratory, Department of Veterinary Medicine, Faculty of Veterinary Medicine, Ahmadu Bello University, P.M.B. 1013, Zaria, Kaduna State, Nigeria.
3College of Agriculture, Ministry of Higher Education, Bauchi State, Nigeria.
4Department of Veterinary Medicine, Faculty of Veterinary Medicine, Ahmadu Bello University, P.M.B.1013, Zaria, Kaduna State, Nigeria.
5Department of Obstetrics and Gyenecology, University of Maiduguri Teaching Hospital, Maiduguri, Borno State, Nigeria.
A. S. Sa’idu1*, E. C. Okolocha1, A. A. Dzikwi1, J. K. P. Kwaga1, A. Usman2, A. A. Gamawa3, U. B. Abubakar4 and S. A. Maigari5
1Department of Veterinary Public Health and Preventive Medicine, Ahmadu Bello University, P.M.B. 1013, Zaria, Kaduna State, Nigeria.
(1) Jimmy T. Efird, Department of Public Health, Epidemiology and Outcomes Research and East Carolina Heart Institute, Brody School of Medicine, Greenville, North Carolina, USA.
(1) Travería Gabriel Eduardo, Veterinary Science Department, La Plata University, Argentina.
(2) Anonymous, University of Zambia, Zambia.
(3) Anonymous, University College Dublin (UCD), Ireland.
Complete Peer review History: http://www.sciencedomain.org/review-history/6730
Aims: Bovine tuberculosis (bTB) is a chronic infectious and contagious zoonotic disease of domestic, wild animals and humans. The disease occurs in a wide range of mammalian species and therefore, poses a public health threat. It also results in considerable economic losses in livestock production and carcass condemnation of infected cattle during meat inspection. This study was aimed at determining the prevalence of zoonotic bovine tuberculosis in slaughtered cattle, based on Post-Mortem (PM) meat inspection, Ziehl-Neelsen staining (ZN) and Polymerase Chain Reaction (PCR) techniques in abattoir and slaughter houses in Bauchi State, Nigeria.
Place and Duration of study: A cross-sectional abattoir based-study was conducted on 800 slaughtered cattle in the Northern, Central and Southern Zonal abattoirs of Bauchi State, Nigeria. This work was carried out between June-September, 2013.
Study Design: Experimental.
Methodology: One hundred and twenty (120) tissue samples from different organs were suspected to have bTB lesions at PM 15% (120/800). Out of the samples examined 35 (29.2%) were Acid-Fast Bacilli (AFB) positive; 10 (8.3%) of which were confirmed positive for M. bovis by the confirmatory Polymerase Chain Reaction (PCR).
Results: The present study found the prevalence rates of 3.33% (4/120) and 5.00% (6/120) for males and females, respectively. This gave an overall prevalence of 8.33% for bTB (M. bovis) based on PCR. Bovine TB sex-specific rates were 10.00% (4/40) and 7.50% (6/80) by PCR, in males and females respectively. Female cattle also had a higher prevalence than male cattle but there was no statistically significant association (p>0.05, x2 = 0.218) between the presence of bTB in the tissues sampled and the sex of the cattle. There was a statistically significant association (p<0.05, x2 =7.002, OR=3.363) between detection of bTB in suspected tissues and the age of cattle. Using ZN, cattle aged six (6) years and above had the highest number of positive bTB cases 67.9% (31), while cattle aged 3-5 years old had the lowest 14.81% (4/27). PCR technique, revealed age-specific prevalence rate in cattle aged 6-8 and 9-11 years were 17.07% and 5.77%, respectively. Bauchi zonal abattoir had the highest number of suspected bTB cases (62.5%), followed by Katagum (26.7%) and Misau (10.8%).
Conclusion: High infection rate of bTB was found among cattle sampled in the study area, with a significant prevalence in Bauchi metropolitan abattoir than the other two (2) slaughter houses (Katagum and Misau). This showed that the prevalence of bTB was higher in Bauchi metropolitan abattoir which supplies larger population of the state with beef. These findings also demonstrated that, there is urgent need for public health authorities in the state to intervene.
Abattoirs; Bauchi; cattle; Mycobacterium bovis; prevalence; post-mortem; Polymerase Chain Reaction (PCR).
Full Article - PDF Page 1220-1229
DOI : 10.9734/BJMMR/2015/11650Review History Comments