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British Biotechnology Journal, ISSN: 2231-2927,Vol.: 4, Issue.: 12 (December)


Effects of Methanolic Extract of Dioclea reflexa Hook F Seed on some Haematological and Kidney Function Parameters in Rats following Single or Repeated Carbon Tetrachloride Intoxication


Sunday Ene-Ojo Atawodi1* and Uju Dorothy Iliemene1

1Biochemistry Department, Ahmadu Bello University, Zaria, Nigeria.

Article Information
(1) Samuel Peña-Llopis, Developmental Biology and Internal Medicine, Oncology Division, University of Texas Southwestern Medical Center, USA.
(1) Anonymous, Helwan University, Egypt.
(2) Yousif Y. Bilto, Dept. Biol and Biomed Sciences, The University of Jordan, Amman, Jordan.
Complete Peer review History: http://www.sciencedomain.org/review-history/6726


Objective: To investigate the effect of methanolic extract of Dioclea reflexa Hook F seed on male rats following single (acute) or repeated (chronic) carbon tetrachloride intoxication.
Methods: Male albino rats were divided into groups of six each consisting of plant extract only, plant extract + carbon tetrachloride, solvent only, solvent + carbon tetrachloride, vitamin E only, vitamin E + carbon tetrachloride and untreated control. The rats in the acute experiment received the extract (5mg/kg) by intraperitoneal injection for two days, and 1 hour after administration of CCl4 (0.6ml/kg body weight) on the third day, while those in the chronic experimental model received 2.5mg/kg for 10 consecutive days with 72 hourly administration of CCl4 at 0.3ml/kg.
Results: In the acute model, the level of total bilirubin and conjugated bilirubin were significantly (P<0.05) reduced in D. reflexa seed extract pre-treated rats compared to the CCl4 control, while in the chronic model, the level of packed cell volume (PCV) and hemoglobin were significantly (P<0.05) boosted in extract treated group compared to the CCl4 control group with concomitant reduction in the levels of bilirubin.
Conclusion: These results indicate that the seed of D. reflexa possess capacity to boost haematological parameters and protect the kidney against acute and chronic toxicological challenges.

Keywords :

Dioclea reflexa; haematological effect; kidney damage; CCl4 intoxication; acute toxicity; chronic toxicity.

Full Article - PDF    Page 1253-1262

DOI : 10.9734/BBJ/2014/13836

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