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British Biotechnology Journal, ISSN: 2231-2927,Vol.: 4, Issue.: 12 (December)

Original-research-article

Effects of Methanolic Extract of Dioclea reflexa Hook F Seed on some Haematological and Kidney Function Parameters in Rats following Single or Repeated Carbon Tetrachloride Intoxication

 

Sunday Ene-Ojo Atawodi1* and Uju Dorothy Iliemene1

1Biochemistry Department, Ahmadu Bello University, Zaria, Nigeria.

Article Information
Editor(s):
(1) Samuel Peña-Llopis, Developmental Biology and Internal Medicine, Oncology Division, University of Texas Southwestern Medical Center, USA.
Reviewers:
(1) Anonymous, Helwan University, Egypt.
(2) Yousif Y. Bilto, Dept. Biol and Biomed Sciences, The University of Jordan, Amman, Jordan.
Complete Peer review History: http://www.sciencedomain.org/review-history/6726

Abstracts

Objective: To investigate the effect of methanolic extract of Dioclea reflexa Hook F seed on male rats following single (acute) or repeated (chronic) carbon tetrachloride intoxication.
Methods: Male albino rats were divided into groups of six each consisting of plant extract only, plant extract + carbon tetrachloride, solvent only, solvent + carbon tetrachloride, vitamin E only, vitamin E + carbon tetrachloride and untreated control. The rats in the acute experiment received the extract (5mg/kg) by intraperitoneal injection for two days, and 1 hour after administration of CCl4 (0.6ml/kg body weight) on the third day, while those in the chronic experimental model received 2.5mg/kg for 10 consecutive days with 72 hourly administration of CCl4 at 0.3ml/kg.
Results: In the acute model, the level of total bilirubin and conjugated bilirubin were significantly (P<0.05) reduced in D. reflexa seed extract pre-treated rats compared to the CCl4 control, while in the chronic model, the level of packed cell volume (PCV) and hemoglobin were significantly (P<0.05) boosted in extract treated group compared to the CCl4 control group with concomitant reduction in the levels of bilirubin.
Conclusion: These results indicate that the seed of D. reflexa possess capacity to boost haematological parameters and protect the kidney against acute and chronic toxicological challenges.

Keywords :

Dioclea reflexa; haematological effect; kidney damage; CCl4 intoxication; acute toxicity; chronic toxicity.

Full Article - PDF    Page 1253-1262

DOI : 10.9734/BBJ/2014/13836

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