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Advances in Research, ISSN: 2348-0394,Vol.: 3, Issue.: 3


The Simultaneous Determination of Some Water-Soluble Vitamins in Gum of Acacia nilotica by High Performance Liquid Chromatography


Sarra Bouazizi1*, Bassem Jamoussi1 and Dalila Bousta2

1Institute of Higher and Continuing Education, Laboratory of Chemical Analysis, 43 Liberty Street, 2019 Bardo, Tunis, Tunisia.
2National Institute of Aromatic Medicinal Plants, BP 7048 - Ez-Kalaa, 7048, Fez, Morocco.


Article Information
(1) Anonymous.
(1) Anonymous, University for Development Studies, Ghana.
(2) Anonymous, Refik Saydam Hygiene Central, Turkey.
(3) Gerardo Díaz Godínez, Autonomous University of Tlaxcala, Tlaxcala, Mexico.
Complete Peer review History: http://www.sciencedomain.org/review-history/6674




A rapid, simple and precise method by HPLC (high performance liquid chromatography) has been developed for simultaneous determination of water-soluble vitamins as thiamine(B1), nicotinamide(B3), panthotenic(B5), pyridoxine(B6) and biotin(B8) in gum of Acacia nilotica using enzymatic hydrolysis. The method uses a C18 column (4.6×150 mm, 5µm). Mobile phase such as methanol 0.1M, sodium dihydrogen phosphate (pH = 2.5), (10:90 v/v) is found most suitable for rapid separation and identification of this water –soluble vitamins. Good linearity was observed between the concentration of analytes and peak area (r = 0.9999). Each vitamin was quantitatively determined at its maximum wavelength. Recovery percentages ranged from 97% to 99%.


Keywords :

Water; soluble vitamins; gum, Acacia nilotica; HPLC.


Full Article - PDF    Page 312-318    Article Metrics


DOI : 10.9734/AIR/2015/10976

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