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Advances in Research

Advances in Research, ISSN: 2348-0394,Vol.: 3, Issue.: 3

Original-research-article

The Simultaneous Determination of Some Water-Soluble Vitamins in Gum of Acacia nilotica by High Performance Liquid Chromatography

 

Sarra Bouazizi1*, Bassem Jamoussi1 and Dalila Bousta2

1Institute of Higher and Continuing Education, Laboratory of Chemical Analysis, 43 Liberty Street, 2019 Bardo, Tunis, Tunisia.
2National Institute of Aromatic Medicinal Plants, BP 7048 - Ez-Kalaa, 7048, Fez, Morocco.

 

Article Information
Editor(s):
(1) Anonymous.
Reviewers:
(1) Anonymous, University for Development Studies, Ghana.
(2) Anonymous, Refik Saydam Hygiene Central, Turkey.
(3) Gerardo Díaz Godínez, Autonomous University of Tlaxcala, Tlaxcala, Mexico.
Complete Peer review History: http://www.sciencedomain.org/review-history/6674

 

Abstracts

 

A rapid, simple and precise method by HPLC (high performance liquid chromatography) has been developed for simultaneous determination of water-soluble vitamins as thiamine(B1), nicotinamide(B3), panthotenic(B5), pyridoxine(B6) and biotin(B8) in gum of Acacia nilotica using enzymatic hydrolysis. The method uses a C18 column (4.6×150 mm, 5µm). Mobile phase such as methanol 0.1M, sodium dihydrogen phosphate (pH = 2.5), (10:90 v/v) is found most suitable for rapid separation and identification of this water –soluble vitamins. Good linearity was observed between the concentration of analytes and peak area (r = 0.9999). Each vitamin was quantitatively determined at its maximum wavelength. Recovery percentages ranged from 97% to 99%.

 

Keywords :

Water; soluble vitamins; gum, Acacia nilotica; HPLC.

 

Full Article - PDF    Page 312-318    Article Metrics

 

DOI : 10.9734/AIR/2015/10976

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