Journal of Advances in Biology & Biotechnology, 2394-1081,Vol.: 2, Issue.: 1
N-alkane Solvent-enhanced Biotransformation of Steroid DHEA by Beauveria bassiana as Biocatalyst
Richard Gonzalez1, Felipe Nicolau1 and Tonya L. Peeples1* 1Department of Chemical and Biochemical Engineering, the University of Iowa 4133 Seamans Center, the University of Iowa, Iowa City, IA. 52242-1527, USA.
Richard Gonzalez1, Felipe Nicolau1 and Tonya L. Peeples1*
1Department of Chemical and Biochemical Engineering, the University of Iowa 4133 Seamans Center, the University of Iowa, Iowa City, IA. 52242-1527, USA.
(1) Marli Camassola, University of Caxias do Sul, Institute of Biotechnology, Brazil.
(1) Anonymous, Unesp, São José do Rio Preto/SP, Brazil.
(2) Anonymous, Gazi University, Turkey.
Complete Peer review History: http://www.sciencedomain.org/review-history/6600
Aims: The hydroxylation capacity of Beauveria bassiana was enhanced with n-alkane solvents, resulting in a selective and eco-friendly scheme for the synthesis of steroids. A biocatalytic system was engineered to augment the 11α-hydroxylation of dehydroepiandrosterone (DHEA) to valuable intermediates. Exposing and inducing cells into n-alkanes improved the synthesis of 11α-hydroxy derivatives.
Methodology and Results: Reactions were carried out with cells grown with n-dodecane (n-C12) and n-hexadecane (n-C16), resulting in 65%±6.3 conversion of DHEA to androstenediol (40.3%mM) and 3β,11α,17β-trihydroxyandrost-5-ene (22.8%mM), as determined by HPLC and NMR analyses. Experiments without the presence of n-alkanes resulted in 17% conversion of DHEA. Isolated products in this case included: Androstenediol (11.8%mM) and 3β,11α,17β-trihydroxyandrost-5-ene (4.78%mM). Results indicate that only the 3,17-hydroxy derivatives of DHEA undergo the 11α-hydroxylation pathway.
Conclusions: The appearance of the products suggests that the reduction of the C-17 ketone of DHEA is preceded by the 11α-hydroxylation reaction when n-alkanes are present. This differs from reports in the literature, which proposed the activation of an unfunctionalized carbon to 11α-hydroxy-17-oxo derivatives before obtaining a 3β,11α,17β-triol product.
Beauveria bassiana; DHEA; steroid biotransformation; bio hydroxylations; biocatalytic reactions; microbial transformation.
Full Article - PDF Page 30-37
DOI : 10.9734/JABB/2015/13541Review History Comments