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British Microbiology Research Journal, ISSN: 2231-0886,Vol.: 5, Issue.: 2

Original-research-article

Multiple-Antibiotic Resistance in Salmonella enterica Serovars Isolated in Iran Harboring Class 1 Integrons

 

Bahareh Rajaei1,2, Nahid Sepehri Rad1, Farzad Badmasti1, Mohamad Reza Razavi3, Mohammad Reza Aghasadeghi4, Raheleh Saboohi1, Taraneh Rajaei5, Arfa Moshiri1,6 and Seyed Davar Siadat1,7*

1Department of Microbiology, Pasteur Institute of Iran, Tehran, Iran.
2Department of Stem Cells, Division of Nanobiomaterials and Tissue Engineering, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran.
3Department of Parasitology, Pasteur Institute of Iran, Tehran, Iran.
4Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran.
5Department of Biotechnology, Pasteur Institute of Iran, Tehran, Iran.
6Research Institute for Endocrine Science, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
7Department of Mycobacteriology and Lung Research, Pasteur Institute of Iran, Tehran, Iran.

Article Information
Editor(s):
(1) Rashedul Islam, Department of Biological Sciences, Inha University, South Korea.
Reviewers:
(1) Anonymous, Umm Al-Qura University, Saudi Arabia.
(2) Anonymous, Universidade Estadual de Londrina, Londrina, Brazil.
Complete Peer review History:http://www.sciencedomain.org/review-history/6392

Abstracts

Aims: This research was carried out to detect the content and distribution of class 1 integrons in multidrug resistant Salmonella isolates.
Materials and Methods: Eighty four clinical isolates of Salmonella serovars were subjected to molecular detection of class 1 integrons following the antimicrobial susceptibility test using disk diffusion method and MIC determination.
Results: Eleven isolates (13.1%) which were resistant to at least 4 groups of antimicrobial agents considered as MDR (multidrug resistant) Salmonella serovars. The intI1 gene and internal variable regions (IVRs) of class 1 integron were detected in 50 (59.5%) and 35 (70%) of Salmonella clinical isolates respectively. Analysis of the sequence data revealed four gene cassette arrays including the dhfr7 (0.8 kb), aadA1 (1kb), blaP1 (1.2 kb), dhfr1-aadA1 (1.6 kb) with eight IVR distribution patterns.
Conclusion: Detection of class 1 integron carrying gene cassettes which confer resistance to different classes of antibiotics such as aminoglycosides, ß-lactams and trimethoprim confirms that integron-mediated antimicrobial gene cassettes are prevalent in Salmonella serovars isolated in Iran.

Keywords :

Class 1 integron; multidrug resistance; gene cassette array.

Full Article - PDF    Page 186-193

DOI : 10.9734/BMRJ/2015/9303

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