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British Journal of Medicine and Medical Research, ISSN: 2231-0614,Vol.: 4, Issue.: 36 (21-31 December)

Original-research-article

Atherosclerosis Vaccine Using Bacteria Salmonella typhimurium on Rat Models

 

S. Winarsih1*, R. B. N. Yudhinata2, M. P. Zaka2, T. S. Luthfia2, F. Tamara2 and A. M. Indra2

1Microbiology Department, Faculty of Medicine, University of Brawijaya, Indonesia.
2Medical Program, Faculty of Medicine, University of Brawijaya, Indonesia.

Article Information

Editor(s):

(1) Jimmy T. Efird, Associate Professor, Department of Public Health, Director of Epidemiology and Outcomes Research, East Carolina Heart Institute, Brody School of Medicine, Greenville, North Carolina, USA.

Reviewers:

(1) Marcio Viana Ramos, Federal University of Ceará, Brazil.

(2) Nadia Sharif, Lahore College for Women University, Pakistan.

(3) Anonymous

Complete Peer review History: http://www.sciencedomain.org/review-history/5815

Abstracts

Aims: To prove the effect of S. typhimurium vaccine on inhibiting foam cell formation and arterial wall thickness, and also to decrease body weight and abdominal visceral fat.
Study Design: This experimental research was conducted using rat models.
Place and Duration of Study: Faculty of Medicine, Brawijaya University, Indonesia, between February – May 2011.
Methodology: The vaccine was 108 CFU of heat-killed S. typhimurium/100µl vaccine per rat. The adjuvant was CFA-IFA 100µl per rat. Twenty Wistar rats were divided into five groups: a negative control group (have normal diet), and four treatment groups which were given with atherogenic diet. The four treatment groups were positive control group (atherogenic diet only), vaccine + adjuvant group (added with the vaccine + adjuvants), vaccine group (added with vaccine only), and adjuvant group (added with adjuvant only). The vaccines were injected intraperitoneally, five times in two-week intervals.
Results: There was no significantly difference in the average diet intake every day among the groups (P=0.17). The administration of ‘vaccine + adjuvants’, ‘vaccine only’ and ‘adjuvants only’ could decrease foam cell formation and arterial wall thickness compared to the positive control group (P= .00). The ‘vaccine alone’ treatment returned the foam cell numbers to be a normal value just like negative control (P=.15), but ‘vaccine + adjuvants ‘and ‘adjuvant alone’ did not (P=.01). There was a strong and significantly correlation between the foam cell formation with arterial wall thickness (R=0.842, P=.00). In addition, administration of ‘vaccine only’ decreased the rats’ body weight and abdominal visceral fat accumulation significantly compared to the positive control (P=.04 and P=.00 respectively).
Conclusion: The heat-killed Salmonella typhimurium vaccine without CFA-IFA adjuvant decreases foam cells expression and aortic wall thickness, body weight, and abdominal visceral fat accumulation in rat-induced atherogenic diet. In suggestion, heat-killed S. typhimurium is a potential antigen to be developed as an atherosclerosis vaccine in the future.

Keywords :

Vaccine; S. typhimurium; foam cell; arterial wall; body weight; abdominal fat.

Full Article - PDF    Page 5729-5740

DOI : 10.9734/BJMMR/2014/4480

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