British Biotechnology Journal, ISSN: 2231-2927,Vol.: 4, Issue.: 7 (July)
In-vitro Propagation of Aloe vera (L.) Burm. f
Sharmistha Gupta1, Pankaj K. Sahu2*, Devki L. Sen3 and Priyanka Pandey2 1West Bengal State Council of Science & Technology, DSTGo WB, Kolkata, West Bengal, India.
2Department of Botany, Dr. C.V. Raman University, Bilaspur, (C.G.) India.
3Department of Botany, Government Science College, Raipur, (C.G.) India.
Sharmistha Gupta1, Pankaj K. Sahu2*, Devki L. Sen3 and Priyanka Pandey2
1West Bengal State Council of Science & Technology, DSTGo WB, Kolkata, West Bengal, India.
(1) Yan Juan, Doctorate of Horticultural Crop Breeding, Sichuan Agricultural University, Ya’an, China.
(2) Kuo-Kau Lee, Department of Aquaculture, National Taiwan Ocean University, Keelung, Taiwan.
(2) Margaret M. H. N. Mollel, University of Limpopo, South Africa.
Complete Peer review History: http://www.sciencedomain.org/review-history/5308
Aim: In vitro culture is used for commercial production and is achieved in aseptic condition using different concentration and combination of Plant Growth Regulators (PGR).
Material and Methods: In present work studied the effects of different plant growth regulator singly or in combination in tissue culture of Aloe vera (L.) Burm. f. belongs to the family Liliaceae or Asphodelaceae, used in ayurveda as well as pharmaceutical industry. Murashige and Skoog (MS) media with different combinations and concentration of growth promoters i.e. Auxin (Indole-3-butyric acid (IBA), α-Naphthalene acetic acid (NAA) and Indole-3-acetic acid (IAA) and cytokinin (Benzyl Amino Purine (BAP) were used in this study. Surface sterilization was standardised with HgCl2 with various concentration and time.
Observation and Result: The development of callus type was observed in the MS media supplemented with BAP for best regeneration, IBA for root formation and NAA, was found the best media for root formation (0.5mg/l) were seen to grow onwards from the tenth day of culture and 90% of root formation took place within a span of 3-4 weeks for maximum callus induction. The maximum number of root & shoot produced is 4.8±0.53 with average length of 3.5±0.35cm.
Discussion: The present work deals with in vitro plant growth of Aloe vera through tissue culture for propagation and ex situ conservation. Regenerated plants after acclimatization were transferred to soil and they showed 85% survival. The culture response was maximum in apical buds (100%) and minimum time required is 9.67 days in the same media. The average length of shoots per culture was 4.0±0.16cm. In present study among the three types of auxins, NAA was found to be the best for root induction.
Aloe vera; micropropagation; MS medium; in vitro; plant growth regulator.
DOI : 10.9734/BBJ/2014/9747Review History Comments