European Journal of Medicinal Plants, ISSN: 2231-0894,Vol.: 4, Issue.: 7 (July)
Fibroblast Growth Stimulation, DPPH Antioxidant Assay and Antimicrobial Activities of Funtumia elastica (Preuss) Stapf (Apocynaceae) Leaf Extracts
Samuel N. Osei-Djarbeng1,4*, Sally J. Cutler2, Ronald R. Cutler3 and Olivia Corcoran1 1Medicines Research Group, School of Health, Sports and Bioscience, University of East London, Stratford Campus, Water Lane, London, E15 4LZ, UK.
2Infection and Immunity Research Group, School of Health, Sports and Bioscience, University of East London, Stratford Campus, Water Lane, London, E15 4LZ, UK.
3School of Biological and Chemical Sciences, Queen Mary, University of London, Mile End Road, London, E1 4NS, UK.
4Department of Pharmaceutical Sciences, Faculty of Medicine and Health Sciences, Kumasi Polytechnic, P. O. Box 854, Kumasi, Ghana.
Samuel N. Osei-Djarbeng1,4*, Sally J. Cutler2, Ronald R. Cutler3 and Olivia Corcoran1
1Medicines Research Group, School of Health, Sports and Bioscience, University of East London, Stratford Campus, Water Lane, London, E15 4LZ, UK.
(1) Marcello Iriti, Professor of Plant Biology and Pathology, Department of Agricultural and Environmental Sciences, Milan State University, Italy.
(1) Suzan Matar, The University of Jordan, Jordan.
Complete Peer review History: http://www.sciencedomain.org/review-history/4171
Aims: To investigate the scientific basis for the wound-healing properties of Funtumia elastica (Apocynaceae) leaf extracts using relevant in vitro fibroblast growth stimulation, antimicrobial and DPPH-antioxidant assays.
Place and Duration of Study: School of Health, Sports and Bioscience (Bioscience Laboratories), University of East London in the United Kingdom, between July 2007 and May 2010.
Methodology: Methanolic extract of the leaves, and petroleum ether, ethyl acetate, n-butanol and aqueous fractions partitioned thereof were tested for antimicrobial activities against common wound pathogens (such as Staphylococcus spp, Pseudomonas aeroginosa and Escherichia coli). The Broth dilution method was used to determine the minimum inhibitory concentrations (MIC) of the extracts and fractions. The antioxidant activities were also determined using a 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical assay; whilst the ability to stimulate fibroblast growth was investigated using the MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide) assay.
Results: The n-butanol fraction exhibited the greatest overall activities. It stimulated the growth of fibroblast cells by 28%, and showed MIC range of 0.13 - 1.0 mg/mL against the Staphylococci species, P. aeruginosa, Bacillus subtilis and E. coli. The non-polar petroleum ether fraction exhibited MICs greater than 2.0 mg/mL against all the organisms. All the fractions exhibited antioxidant activities greater than or comparable to that of ascorbic acid.
Conclusion: Collectively, the antioxidant activity, fibroblast growth stimulation and the antimicrobial activities demonstrated by F. elastica leaf extracts provide some evidence to support the use of the plant to manage wounds in African folklore medicine.
Wound-healing; Funtumia elastica; fibroblast; antioxidant; antimicrobial; DPPH.
Full Article - PDF Page 835-843
DOI : 10.9734/EJMP/2014/9316Review History Comments