European Journal of Medicinal Plants, ISSN: 2231-0894,Vol.: 4, Issue.: 6 (June)
Antioxidant and Antibacterial Activity of Field Grown and Tissue Cultured Root Callus of Mangrove Species
Chinnappan Ravinder Singh1*, Kandasamy Kathiresan1, Sekar Anandhan1 and Kanagaraj Suganthi1 1Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai 608 502, Tamil Nadu, India.
Chinnappan Ravinder Singh1*, Kandasamy Kathiresan1, Sekar Anandhan1 and Kanagaraj Suganthi1
1Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai 608 502, Tamil Nadu, India.
(1) Marcello Iriti, Professor of Plant Biology and Pathology, Department of Agricultural and Environmental Sciences, Faculty of Agricultural and Food Sciences, Milan State University, Via G. Celoria 2 - 20133 Milan, Italy.
(1) María Luisa Kennedy, Universidad Nacional De Asunción, Paraguay.
(2) Raj Narayan Roy, Dr. B N Dutta Smriti Mahavidyalaya Hatgobindapur, India.
(3) M. Ángeles Ramírez-Cisneros, Universidad Autónoma del Estado de Morelos, México.
Complete Peer review History: http://www.sciencedomain.org/review-history/3879
Aim: The main aim of this study is to evaluate the anti-oxidant and antimicrobial activity of mangrove species and the development of the callus biomass.
Study Design: This is the first comparative evaluation report of field grown root and tissue cultured root callus of mangrove on anti-oxidant and anti-bacterial activity. Acanthus ilicifolius, Calophyllum inophyllum and Excoecaria agallocha were tested in this present investigation.
Place and Duration of Study: Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai 608 502, Tamil Nadu, India. Between February 2012 and July 2013
Methodology: In order to develop callus biomass, a number of growth hormones were supplemented with the MS medium. Following the callus development, anti-oxidant and anti-microbial activities were tested with field grown root and its tissue cultured root callus of Acanthus ilicifolius, Calophyllum inophyllum and Excoecaria agallocha. This was confirmed by different anti-oxidant, anti-bacterial and minimum inhibitory concentration assays.
Results: Maximum (89%) root callus biomass was obtained from Acanthus ilicifolius on MS medium fortified with 0.3+0.3 mg/L of 2,4-D and KIN. 2,4-D 0.3mg/L and BAP 0.5 mg/L showed the maximum callus from both Calophyllum inophyllum (81%), Excoecaria agallocha (58%). In addition anti-oxidant and anti-bacterial effect of root and root callus of these three species were tested. In this study the root callus materials of all the three species showed the best anti-oxidant anti-bacterial activities.
Conclusion: The metabolites from mangroves are good remedy for number of health problems especially the enhanced level of metabolites through the tissue culture techniques. The present study confirmed the anti-oxidant and anti-bacterial effect of Acanthus ilicifolius, Calophyllum inophyllum, Excoecaria agallocha n root extract. This study will be a key to develop a new drug to achieve healthy life. especially the root callus materials showed the better activity when compared to the grown plants.
Callus biomass; Acanthus ilicifolius; Calophyllum inophyllum; Excoecaria agallocha; anti-microbial; resazurin method; anti-oxidant.
Full Article - PDF Page 723-742
DOI : 10.9734/EJMP/2014/7340Review History Comments