European Journal of Medicinal Plants, ISSN: 2231-0894,Vol.: 4, Issue.: 6 (June)

Original-research-article

Antioxidant and Antibacterial Activity of Field Grown and Tissue Cultured Root Callus of Mangrove Species

 

Chinnappan Ravinder Singh^1*, Kandasamy Kathiresan¹, Sekar Anandhan¹ and Kanagaraj Suganthi¹

¹Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai 608 502, Tamil Nadu, India.

Article Information

Editor(s):

(1) Marcello Iriti, Professor of Plant Biology and Pathology, Department of Agricultural and Environmental Sciences, Faculty of Agricultural and Food Sciences, Milan State University, Via G. Celoria 2 - 20133 Milan, Italy.

Reviewers:

(1) María Luisa Kennedy, Universidad Nacional De Asunción, Paraguay.

(2) Raj Narayan Roy, Dr. B N Dutta Smriti Mahavidyalaya Hatgobindapur, India.

(3) M. Ángeles Ramírez-Cisneros, Universidad Autónoma del Estado de Morelos, México.

Complete Peer review History: http://www.sciencedomain.org/review-history/3879

Aim: The main aim of this study is to evaluate the anti-oxidant and antimicrobial activity of mangrove species and the development of the callus biomass.
Study Design: This is the first comparative evaluation report of field grown root and tissue cultured root callus of mangrove on anti-oxidant and anti-bacterial activity. Acanthus ilicifolius, Calophyllum inophyllum and Excoecaria agallocha were tested in this present investigation.
Place and Duration of Study: Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai 608 502, Tamil Nadu, India. Between February 2012 and July 2013
Methodology: In order to develop callus biomass, a number of growth hormones were supplemented with the MS medium. Following the callus development, anti-oxidant and anti-microbial activities were tested with field grown root and its tissue cultured root callus of Acanthus ilicifolius, Calophyllum inophyllum and Excoecaria agallocha. This was confirmed by different anti-oxidant, anti-bacterial and minimum inhibitory concentration assays.
Results: Maximum (89%) root callus biomass was obtained from Acanthus ilicifolius on MS medium fortified with 0.3+0.3 mg/L of 2,4-D and KIN. 2,4-D 0.3mg/L and BAP 0.5 mg/L showed the maximum callus from both Calophyllum inophyllum (81%), Excoecaria agallocha (58%). In addition anti-oxidant and anti-bacterial effect of root and root callus of these three species were tested. In this study the root callus materials of all the three species showed the best anti-oxidant anti-bacterial activities.
Conclusion: The metabolites from mangroves are good remedy for number of health problems especially the enhanced level of metabolites through the tissue culture techniques. The present study confirmed the anti-oxidant and anti-bacterial effect of Acanthus ilicifolius, Calophyllum inophyllum, Excoecaria agallocha n root extract. This study will be a key to develop a new drug to achieve healthy life. especially the root callus materials showed the better activity when compared to the grown plants.

Keywords :

Callus biomass; Acanthus ilicifolius; Calophyllum inophyllum; Excoecaria agallocha; anti-microbial; resazurin method; anti-oxidant.

Full Article - PDF    Page 723-742

DOI : 10.9734/EJMP/2014/7340

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