British Journal of Applied Science & Technology, ISSN: 2231-0843,Vol.: 4, Issue.: 10 (01-10 April)
Fermentation Optimization of Mycobacterium smegmatis Using Experimental Design
Sebastian O. Wendel1,3, Ayomi S. Perera2, Peter H. Pfromm3, Peter Czermak1,3 and Stefan H. Bossmann2* 1Technische HochschuleMittelhessen, Department of Biotechnology/Biopharmaceutical Technology, Wiesenstrasse 14, 35390 Giessen, Germany.
2Kansas State University, Department of Chemistry, Manhattan, 201 CBC Building, KS 66506-0401, United States.
3Kansas State University, Department of Chemical Engineering, 1036 Durland Hall, Manhattan, KS 66506, United States.
Sebastian O. Wendel1,3, Ayomi S. Perera2, Peter H. Pfromm3, Peter Czermak1,3 and Stefan H. Bossmann2*
1Technische HochschuleMittelhessen, Department of Biotechnology/Biopharmaceutical Technology, Wiesenstrasse 14, 35390 Giessen, Germany.
(1) Christo Z. Christov, Senior Lecturer in Computational Biochemistry, Department of Applied Sciences, Faculty of Health and Life Sciences, Northumbria University, Ellison Building, Newcastle-upon-Tyne, NE1 8ST, UK.
Complete Peer review History: http://www.sciencedomain.org/review-history/3552
The exceptionally stable mycobacterial protein porin A (MspA) from Mycobacterium smegmatis has potential applications in protein-based solar cells, and as a biotemplate for nano-wires and nano-dots. These applications would be enabled by an efficient and cost effective method to grow the host organism at high cell mass yields, and recover purified MspA.
In this work, the cell mass yield was maximized and costs lowered by applying experimental design (varying nitrogen and iron contents according to a Doehlert matrix) based on a minimal fermentation medium that was reported earlier. Glucose use was minimized by adjusting glucose feed based on analyzing residual glucose after fermentation. The costs for extracted and purified MspA were lowered by 67% for the minimum medium and the optimized composition derived here compared to commercial medium (7H9 Middlebrook).
Mycobacterium smegmatis; MspA; medium optimization; protein extraction.
Full Article - PDF Page 1472-1484
DOI : 10.9734/BJAST/2014/5702Review History Comments