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British Journal of Medicine and Medical Research, ISSN: 2231-0614,Vol.: 4, Issue.: 11 (11-20 April)

Original-research-article

Evaluation of Antioxidant and Acteylcholinesterase-inhibitory Properties of Methanol Extracts of Nauclealatifolia, Cymbopogon citratus and Cocos nucifera: An In vitro Study

 

Oluwatosin Adekunle Adaramoye1* and Fausat Adesola Azeez1

1Drug Metabolism and Toxicology Research Laboratories, Department of Biochemistry, College of Medicine, University of Ibadan, Ibadan, Nigeria.

Article Information

Editor(s):

(1) Claudia Borza, “Victor Babes” University of Medicine and Pharmacy, Department of Pathophysiology, România.

Reviewers:

(1) Anonymous.

(2) Lim Vuanghao, Advanced Medical and Dental Institute, Universiti Sains Malaysia, Malaysia.

(3) Anonymous.

(4) Anonymous.

(5) Anonymous.

Complete Peer review History: http://www.sciencedomain.org/review-history/3422

Abstracts

Aims: To evaluate the in vitro antioxidant and acetylcholinesterase (AChE)-inhibitory potentials of methanol extracts of Nauclea latifolia (NL), Cymbopogon citratus (CC) and Cocos nucifera (CN).
Study Design/Methodologies: The antioxidant and AChE- inhibition activities were evaluated using standard in vitro methods viz; DPPH (2,2-diphenyl-1-picrylhydrazine), nitric oxide (NO.), hydroxyl radical (OH.) and hydrogen peroxide (H2O2) radical scavenging assays as well as reducing power, Fe2+/ascorbate-induced lipid peroxidation (LPO) and AChE inhibition assays.
Place and Duration of the Study: The study and analyses were carried out at the Department of Biochemistry, University of Ibadan between March and June 2012.
Results: Extract of NL has the highesttotal phenol and flavonoids contents. The antioxidant activities of the extracts followed the order; CN> NL> CC. Based on DPPH radical scavenging, extract of CN was the most effective. The DPPH scavenging potential of CN, NL and CC were 88%, 82% and 76%, respectively relative to catechin (standard) (91%). The IC50 for the scavenging of hydroxyl radicals by CN, CC and NL were 145.3, 148.8 and 162.3 μg/mL, respectively while catechin is 178.6μg/mL. The reducing powers of CN and NL were statistically similar to catechin. At100 μg/mL, extracts of CN, CC and NL inhibited hepatic LPO by 41%, 22% and 29% respectively. Importantly, extract of CN significantly (p<0.05) inhibited brain LPO and promotes NO. scavenging by 48% and 23%, respectively. Also, CN at 250 and 500 μg/mL significantly (p<0.05) inhibited AChE activities by 33% and 75%, respectively.
Conclusion: CC, NL and CN exhibited strong antioxidant activities but only CN has significant AChE-inhibitory potential.

Keywords :

Acteylcholinesterase; antioxidant; free radicals; lipid peroxidation.

Full Article - PDF    Page 2156-2170

DOI : 10.9734/BJMMR/2014/7206

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