British Journal of Medicine and Medical Research, ISSN: 2231-0614,Vol.: 4, Issue.: 11 (11-20 April)
Evaluation of Antioxidant and Acteylcholinesterase-inhibitory Properties of Methanol Extracts of Nauclealatifolia, Cymbopogon citratus and Cocos nucifera: An In vitro Study
Oluwatosin Adekunle Adaramoye1* and Fausat Adesola Azeez1 1Drug Metabolism and Toxicology Research Laboratories, Department of Biochemistry, College of Medicine, University of Ibadan, Ibadan, Nigeria.
Oluwatosin Adekunle Adaramoye1* and Fausat Adesola Azeez1
1Drug Metabolism and Toxicology Research Laboratories, Department of Biochemistry, College of Medicine, University of Ibadan, Ibadan, Nigeria.
(1) Claudia Borza, “Victor Babes” University of Medicine and Pharmacy, Department of Pathophysiology, România.
(2) Lim Vuanghao, Advanced Medical and Dental Institute, Universiti Sains Malaysia, Malaysia.
Complete Peer review History: http://www.sciencedomain.org/review-history/3422
Aims: To evaluate the in vitro antioxidant and acetylcholinesterase (AChE)-inhibitory potentials of methanol extracts of Nauclea latifolia (NL), Cymbopogon citratus (CC) and Cocos nucifera (CN).
Study Design/Methodologies: The antioxidant and AChE- inhibition activities were evaluated using standard in vitro methods viz; DPPH (2,2-diphenyl-1-picrylhydrazine), nitric oxide (NO.), hydroxyl radical (OH.) and hydrogen peroxide (H2O2) radical scavenging assays as well as reducing power, Fe2+/ascorbate-induced lipid peroxidation (LPO) and AChE inhibition assays.
Place and Duration of the Study: The study and analyses were carried out at the Department of Biochemistry, University of Ibadan between March and June 2012.
Results: Extract of NL has the highesttotal phenol and flavonoids contents. The antioxidant activities of the extracts followed the order; CN> NL> CC. Based on DPPH radical scavenging, extract of CN was the most effective. The DPPH scavenging potential of CN, NL and CC were 88%, 82% and 76%, respectively relative to catechin (standard) (91%). The IC50 for the scavenging of hydroxyl radicals by CN, CC and NL were 145.3, 148.8 and 162.3 μg/mL, respectively while catechin is 178.6μg/mL. The reducing powers of CN and NL were statistically similar to catechin. At100 μg/mL, extracts of CN, CC and NL inhibited hepatic LPO by 41%, 22% and 29% respectively. Importantly, extract of CN significantly (p<0.05) inhibited brain LPO and promotes NO. scavenging by 48% and 23%, respectively. Also, CN at 250 and 500 μg/mL significantly (p<0.05) inhibited AChE activities by 33% and 75%, respectively.
Conclusion: CC, NL and CN exhibited strong antioxidant activities but only CN has significant AChE-inhibitory potential.
Acteylcholinesterase; antioxidant; free radicals; lipid peroxidation.
Full Article - PDF Page 2156-2170
DOI : 10.9734/BJMMR/2014/7206Review History Comments