British Journal of Medicine and Medical Research, ISSN: 2231-0614,Vol.: 2, Issue.: 1 (January-March)
Effect of 67Zn-Nanoparticles on Leukemic Cells and Normal Lymphocytes
Marina A. Orlova1*, Elena Yu. Osipova2 and Sergey A. Roumiantsev2 1Department of Chemistry, M. V. Lomonosov Moscow State University, 1/3, Leninskie Gory, Moscow, 119899, Russian Federation.
2Federal Research Center of Pediatric Hematology, Oncology and Immunology FSI, bd.2, 117, Lenin Ave., Moscow, 117997, Russian Federation.
Marina A. Orlova1*, Elena Yu. Osipova2 and Sergey A. Roumiantsev2
1Department of Chemistry, M. V. Lomonosov Moscow State University, 1/3, Leninskie Gory, Moscow, 119899, Russian Federation.
Porphyrin-fulleren-based nanoparticles (NP), containing magnetic isotopes 25Mg, 67Zn and zinc of natural isotope composition (Zntotal-NP), have been tested on leukemic cells of patients with T-ALL, B-ALL, AML and lymphocytes of healthy donors. Reliable differences in action of magnetic and non-magnetic zinc isotopes for some types of cells were obtained. Magnetic magnesium isotopes and pure nanoparticles of porphyrinfulleren did not demonstrate any effects. 67Zn-NP induced high cytotoxicity in cells of acute B-lymphoblastic leukemia with LD50 almost three times lower, than those for healthy donors, and 4 times lower in comparison with Zntotal-NP. Also evaluation of apoptosis process in granulocytes of healthy donors in the case of the preparates were performed by method of flow cytometry.
Fullerene-based nanoparticles; Lsotopy; 67Zn; 25Mg; apoptosis; MTT-method; flow cytometry;
Full Article - PDF Page 21-30
DOI : 10.9734/BJMMR/2012/783Review History Comments