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Journal of Complementary and Alternative Medical Research, ISSN: 2456-6276,Vol.: 6, Issue.: 2


Alterations in Oral Cancer Gene Expression in Response to Melatonin


Arin Hartounian1, Guillermo Alessandro Retis1, Karl Kingsley2* and Katherine Howard2

1Department of Clinical Sciences, School of Dental Medicine, University of Nevada, Las Vegas, 1700 W. Charleston Blvd., Las Vegas, Nevada, 89106, USA.

2Department of Biomedical Sciences, School of Dental Medicine, University of Nevada, Las Vegas, 1001 Shadow Lane, Las Vegas, Nevada, 89106, USA.

Article Information


(1) Dr. Suma B. V., Assistant professor, Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Ramaiah University of Applied Sciences, Bangalore, India.


(1) Sangeetha A/P Arullappan, Universiti Tunku Abdul Rahman, Malaysia.

(2) Jerónimo Lazos, Universidad Nacional de Córdoba, Argentina.

(3) Glaucia Maria Machado-Santelli, University of So Paulo, Brazil.

Complete Peer review History: http://www.sciencedomain.org/review-history/26652


Aims: Melatonin (MLT) exerts oncostatic effects on numerous tumour types presumably by inhibiting cell proliferation and promoting apoptosis. The primary aim of this study was to investigate melatonin-induced changes in gene expression patterns in two different oral squamous carcinoma cell lines (OSCC).

Methodology: This was a prospective non-randomized experimental study design that was conducted at Department of Biomedical Sciences at the University of Nevada, Las Vegas – School of Dental Medicine between May 2016 and March 2017. The SCC25 and CAL27 cell lines were cultured with and without MLT (10 ug/mL) for 72 hours, and total cellular RNA was isolated and converted to cDNA. The expression of 92 genes associated with the molecular mechanisms of cancer and four endogenous control genes were examined by qRT-PCR. The fold change with respect to control levels were calculated using the comparative method or delta-delta ddCt algorithm.

Results: Gene expression was compared between the untreated and treated cells, SCC25 and CAL27. Although 40% of the genes (n=37/92) in SCC25 cells demonstrated different expression levels, only six were outside the relative-fold change values observed with all other genes (PTEN, MAPK3K5, BCL2, TAGA2B, MAX, and NFKB-IA). In CAL27 cell over 70% (n=65/92) genes exhibited different expression levels, with only two outside the relative fold-change values observed with all other genes (MAPK3K5, FZD1).

Conclusion: MLT administration to oral cancer cells may induce substantial changes in the expression levels of genes associated with the molecular mechanisms of cancer. However, relatively few of these changes were outside the range of observed values. Therefore, continued analysis and verification of these results in other oral cancers may reveal common MLT-induced changed and provide insights into the potential mechanisms of MLT-induced oncostatic effects in oral cancers.


Keywords :

Oral squamous cell carcinoma; cancer gene expression; melatonin.

Full Article - PDF    Page 1-8

DOI : 10.9734/JOCAMR/2018/44008

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