Biotechnology Journal International, 2456-7051,Vol.: 21, Issue.: 4
Use of Palm Male Inflorescence and River-Sand as Acclimatization Substrate for Plantain (Musa sp.) Cultivars
Ekwa Yawa Monono1*, Jemimah Evenye Ngale1, Levai Lewis Dopgima1 and Akongte Peter Njukang1 1Biotechnology Laboratory, Institute of Agricultural Research for Development (IRAD), Ekona, PMB 25 Buea, South West Region, Cameroon.
Ekwa Yawa Monono1*, Jemimah Evenye Ngale1, Levai Lewis Dopgima1 and Akongte Peter Njukang1
1Biotechnology Laboratory, Institute of Agricultural Research for Development (IRAD), Ekona, PMB 25 Buea, South West Region, Cameroon.
(1) Dr. Kazutoshi Okuno, Japan Association for Techno-innovation in Agriculture, Forestry and Fisheries (JATAFF), Japan.
(1) Varaporn Veraplakorn, Ramkhamhaeng University, Thailand.
(2) Jose de Jesus Arellano Garcia, Univesisdad Autonoma del Estado de Morelos, Mexico.
(3) Esin Oluk, Ege University, Turkey.
Complete Peer review History: http://www.sciencedomain.org/review-history/26202
The objective of this work was to investigate the use of Palm Male Inflorescence (PMI) and river-sand as substrate for the acclimatization of plantain. Plantlets from three plantain cultivars (Batard, Ebanga and French Clair) were obtained after 16 weeks of tissue cultures and the plantlets were subjected to routine acclimatization under screen house conditions using two different substrates mixed in different ratios (100% Sand, 100% PMI, 75% PMI, 60% PMI and 50% PMI). The experiment was arranged in a completely randomized design with ten (10) replications; each replicate consisting of one micro-pot. The different substrates used significantly influenced the performance of the cultivars. The best medium for acclimatization for French Clair was 60% PMI in terms of percentage survival of plantlets (96.88%), plantlet height (6.03 cm), diameter (0.60 cm), number of leaves (4.42 leaves), leaf area (20.23 cm2), leaf emergence rate (1.64), number of roots (7.70 roots), and root length (18.86 cm). Ebanga plantlets had the best results with 75% PMI in terms of percentage survival of plantlets (96.88%), plantlet height (6.18 cm), diameter (0.62 cm), number of leaves (4.39 leaves), leaf area (20.48 cm2), leaf emergence rate (1.76), and total fresh weight (10.05 g). Meanwhile with Batard cultivar, 50% PMI was the best substrate in terms of percentage survival of plantlets (96.88%), plantlet height (4.41 cm), diameter (0.55 cm), number of leaves (4.55 leaves), leaf area (12.96 cm2), leaf emergence rate (1.55), and number of roots (5.73 roots). This study clearly show that PMI can be a viable substrate to use with sand in plantlet acclimatization; however, the different plant cultivars had optimal result at different proportions of PMI.
Plantain plantlets; ebanga; french clair; batard; palm male inflorescence; sand; acclimatization.
DOI : 10.9734/BJI/2018/43879Review History Comments