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Journal of Advances in Microbiology, 2456-7116,Vol.: 12, Issue.: 1

Original-research-article

Aerobic Bacteria and Fungi on the Surfaces of a Tertiary Assistance Hospital from Northern Brazil

 

Layane Gabrielle Coelho de Lima1, Michele Alves Sanches2, Ana Cláudia Alves Cortez2 and João Vicente Braga de Souza2*

1Department of Nursing, State University of Amazonas (UEA), Brazil.

2Laboratory of Mycology, National Research Institute of the Amazon (INPA), Brazil.

Article Information

Editor(s):

(1) Dr. Foluso O. Osunsanmi, Department of Biochemistry and Microbiology, University of Zululand, South Africa.

Reviewers:

(1) Alvaro Francisco Lopes de Sousa, University of São Paulo, Brazil.

(2) Iramiot Jacob Stanley, Busitema University, Uganda.

(3) S. Thenmozhi, Department of Microbiology, Periyar University, India.

Complete Peer review History: http://www.sciencedomain.org/review-history/26183

Abstracts

Background and Objectives: It is very common that hospital environment is colonised by microorganisms. This colonisation is a potential threat for hospitalised patients, especially in high-risk services. Quantification of microorganisms from surfaces is an important strategy to control the hospital infection.

Materials and Methods: Total 70 samples were collected and seeded in the Mueller Hinton and Sabouraud Agar culture media. Media cultures in Petri dishes were incubated and microbial growth was quantified (CFU/cm2). Bacteria cultures were characterised by Gram stain. Fungal cultures were submitted for micromorphological evaluation and CHROMagar™ incubation.

Results: Bacterial population higher than 250 CFU/cm2 was found in 11 out of 70 samples. Fungi were found in 3 out of 70 samples. Common manipulation surfaces as water drinking unit, shelf and water tap presented the highest contamination rates. Bacterial cultures (n= 95) showed mostly the presence of Gram-negative bacteria (81%). In addition, regarding the fungal cultures (n= 129) mostly filamentous fungi (72%), from Aspergillus genera were obtained.

Conclusion: Most of the investigated hospital surfaces presented low contamination (< 5 CFU/cm2). However, microbiological studies should be regular in critical areas in order to reinforce measures to control and prevent hospital infection.

Keywords :

Bacteria; fungi; surfaces; hospital.

Full Article - PDF    Page 1-7 Article Metrics

DOI : 10.9734/JAMB/2018/42991

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