Microbiology Research Journal International, 2456-7043,Vol.: 24, Issue.: 4
Production Characteristics and Molecular Properties of Protease of Pediococcus acidilactici Isolated from Beef under Cold Storage
Fadahunsi Ilesanmi Festus1*, Duyilemi Olubunmi Pauline1 and Onilude Abiodun Anthony1 1Department of Microbiology, Faculty of Science, University of Ibadan, Nigeria.
Fadahunsi Ilesanmi Festus1*, Duyilemi Olubunmi Pauline1 and Onilude Abiodun Anthony1
1Department of Microbiology, Faculty of Science, University of Ibadan, Nigeria.
(1) Giuseppe Blaiotta, Professor, Department of Agriculture, Division of “Grape and Wine Sciences”, University of Naples Federico II, Via Universita' 100 – Palazzo Mascabruno, Italy.
(1) Olaolu Oyedeji, Obafemi Awolowo University, Nigeria.
(2) Mina Ilyas, Institute of Molecular Biology and Biotechnology, University of Lahore, Pakistan.
Complete Peer review History: http://www.sciencedomain.org/review-history/25818
Aim: To investigate the production characteristics and molecular properties of protease from Pediococcus acidilactici under cold storage temperature.
Study Design: Identification and re-identification of Pediococcus acidilactis and determination of optimal conditions for protease production and molecular properties.
Place and Duration of Study: All the work was carried out in the Department of Microbiology, Faculty of Science, University of Ibadan, Nigeria between March 2014- January, 2015.
Methodology: Identification of the organism was done using API kit. Determination of inoculums concentration was carried using the Neubaeur chamber. pH, incubation period, substrate concentration, influence of ions, were determined by varying this parameters using standard methods to optimise values. Purification of the enzyme was obtained by gel chromatography using sephadex beads G-100, G-50 and finally by the use of sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE).
Results: The highest protease activity of 49.46 units ml-1 was recorded at pH 5.5, temperature of 20ºC, incubation period of 96 h, inoculum size of 0.1 ml (1.70 x 104) with 1% casein substrate concentration in the presence of 0.1 M manganese ion and 0.1M of Ammonium ions concentration. Separation of protease enzyme on SDS-PAGE showed that the molecular weights of the major peaks were found to be within the range of 45-55 kDa.
Applications: This study revealed that bacteria (Pediococcus acidilactici) could be induced for protease production and their applications in industrial spheres were confirmed.
Pediococcus acidilactici; protease activity; cold-temperature-storage; production characteristics; molecular properties.
DOI : 10.9734/MRJI/2018/32506Review History Comments