Quick Menu

Upcoming Journals

Microbiology Research Journal International

Microbiology Research Journal International, 2456-7043,Vol.: 24, Issue.: 4


Production Characteristics and Molecular Properties of Protease of Pediococcus acidilactici Isolated from Beef under Cold Storage


Fadahunsi Ilesanmi Festus1*, Duyilemi Olubunmi Pauline1 and Onilude Abiodun Anthony1

1Department of Microbiology, Faculty of Science, University of Ibadan, Nigeria.


Article Information


(1) Giuseppe Blaiotta, Professor, Department of Agriculture, Division of “Grape and Wine Sciences”, University of Naples Federico II, Via Universita' 100 – Palazzo Mascabruno, Italy.


(1) Olaolu Oyedeji, Obafemi Awolowo University, Nigeria.

(2) Mina Ilyas, Institute of Molecular Biology and Biotechnology, University of Lahore, Pakistan.

Complete Peer review History: http://www.sciencedomain.org/review-history/25818




Aim: To investigate the production characteristics and molecular properties of protease from Pediococcus acidilactici under cold storage temperature.

Study Design: Identification and re-identification of Pediococcus acidilactis and determination of optimal conditions for protease production and molecular properties.

Place and Duration of Study:  All the work was carried out in the Department of Microbiology, Faculty of Science, University of Ibadan, Nigeria between March 2014- January, 2015.

Methodology: Identification of the organism was done using API kit. Determination of inoculums concentration was carried using the Neubaeur chamber. pH, incubation period, substrate concentration, influence of ions, were determined by varying this parameters using standard methods to optimise values. Purification of the enzyme was obtained by gel chromatography using sephadex beads G-100, G-50 and finally by the use of sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). 

Results: The highest protease activity of 49.46 units ml-1 was recorded at pH 5.5, temperature of 20ºC, incubation period of 96 h, inoculum size of 0.1 ml (1.70 x 104) with 1% casein substrate concentration in the presence of 0.1 M manganese ion and 0.1M of Ammonium ions concentration. Separation of protease enzyme on SDS-PAGE showed that the molecular weights of the major peaks were found to be within the range of 45-55 kDa.

Applications: This study revealed that bacteria (Pediococcus acidilactici) could be induced for protease production and their applications in industrial spheres were confirmed.


Keywords :

Pediococcus acidilactici; protease activity; cold-temperature-storage; production characteristics; molecular properties.


Full Article - PDF    Page 1-14    Article Metrics


DOI : 10.9734/MRJI/2018/32506

Review History    Comments

Search this site

Advanced Search

Announcement & News

Nature (Impact Factor: 41.6) confirmed high standard of SDI journal and its editors

We are happy to inform that Nature (Impact Factor: 41.6) confirmed high standard of SDI journal and ...

ISI Thomson Reuters selected British Journal of Pharmaceutical Research for Emerging Sources Citation Index

We are delighted to inform that ISI Thomson Reuters selected British Journal of Pharmaceutical Resea...

SCOPUS selected Annual Research & Review in Biology (ARRB)

We are delighted to inform that famous indexing organization SCOPUS (from Elsevier) selected  A...

Index Copernicus Evaluation Result Released

We are delighted to inform that Index Copernicus (a leading indexing organization from Pol...

Journal Repository (JR): Permanent Digital Archiving of SDI journals

SDI is happy to announce that all our journals are now permanently archived in Journal Repository (J...

SDI journal got 35th ranking in Publons

We are delighted to announce (as of 04/01/2016) that British Journal of Medicine and Medical Re...

Growth of SDI and world publication market

As of 2014, total 25,064 journals are competing in World market of journal publication. In 2011, tot...

Science (IF: 31) report confirmed the high standard of SDI journal

As per a recent report (Link) of Science journal (present Impact factor 31), one of our journal (Bri...

SDI introduced Post-publication peer review by its comment section

SDI journals encourage Post-publication peer review by its comment section   Policy details a...

SDI promotes transparent Advanced OPEN peer review

We have migrated to transparent and toughest ‘Advanced OPEN peer review’ system (...


  • No Awards listed.

Browser Compatibility : Mozila firefox, Google Crome and IE 7 & above. Creative Commons License Terms & Condition   |   Privacy Policy   |   Join Us   |   Help   |   Contact Us
© Copyright 2010-2018, SCIENCEDOMAIN international. All rights reserved.