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European Journal of Medicinal Plants, ISSN: 2231-0894,Vol.: 24, Issue.: 3

Short Research Article

Castanea sativa Mill. Extract Cytotoxicity


José Manuel Neves1*

1Faculdade de Ciências da Saúde, Universidade Fernando Pessoa, Rua Carlos da Maia, 296 |4200-150 Porto, Portugal.

Article Information


(1) Dr. Shanfa Lu, Professor, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, China.

(2) Dr. Marcello Iriti, Professor, Plant Biology and Pathology, Department of Agricultural and Environmental Sciences, Milan State University, Italy.


(1) Bing Hu, Longhua Hospital Shanghai University of Traditional Chinese Medicine, China.

(2) Mustafa Sevindik, Gaziantep University, Turkey.

Complete Peer review History: http://www.sciencedomain.org/review-history/25686


Castanea sativa Mill. leaves infusion/decoction has been traditionally used in the northern region of Portugal to treat cough in children, diarrhoea, infertility and hypertension. However, no information exists about its possible toxic effects.

This study aims to investigate the toxicity of C. sativa Mill. extract by the determination of population growth impairment, generation time, LC50, morphometric changes in Tetrahymena pyriformis and MTT test.

Generation time, growth and MTT assay are affected by chestnut tree leaves extract and these effects are dosage dependent. The morphometry of the Tetrahymena pyriformis cells was also affected. According to the obtained LC50 value, the extract may be considered as mildly toxic.

The usage of C. sativa leaves extract must be exercised with caution especially when it comes to children.

Keywords :

Castanea sativa Mill.; herbal medicines; toxicity; Tetrahymena pyriformis.

Full Article - PDF    Page 1-4

DOI : 10.9734/EJMP/2018/42780

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