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Journal of Complementary and Alternative Medical Research, ISSN: 2456-6276,Vol.: 5, Issue.: 3

Original-research-article

Phytochemical Screening and in vitro Antiproliferative Activity of the Fruit of Annona muricata and Abelmoschus esculentus Pods against Selected Cancer Cell Lines

 

Mercy Jepkorir1, Tobias Ambundo1, Chrispus Mutuku Ngule2,3, Jecinta Wanjiru Ndungu’4, Douglas Kahura Njuguna5, Regina Wachuka Mbugua2,6, Jean Chepng’etich3 and Peter Githaiga Mwitari2*

1Department of Chemistry and Biochemistry, Laikipia University, P.O.Box 1100, Nyahururu, Kenya.

 2Centre for Traditional Medicine and Drug Research, Kenya Medical Research Institute, P.O.Box 54840-00200, Nairobi, Kenya.

3Department of Biochemistry, Jomo Kenyatta University of Agriculture and Technology, P.O.Box 62000- 00200, Nairobi, Kenya.

4Department of Biochemistry and Molecular Biology, Egerton University, P.O.Box 536, Egerton, Kenya.

5Department of Biochemistry, Rongo University, P.O.Box 103-40404, Rongo, Kenya.

6Department of Biochemistry and Biotechnology, Kenyatta University, P.O.Box 43844-00100, Nairobi, Kenya.

Article Information

Editor(s):

(1) Sahdeo Prasad, Department of Experimental Therapeutics, The University of Texas MD Anderson Cancer Center, Texas, USA.

Reviewers:

(1) M. V. N. L. Chaitanya, Guru Nanak School of Pharmacy, India.

(2) Sangeetha A/P Arullappan, Universiti Tunku Abdul Rahman (UTAR), Malaysia.

(3) Jolanta Flieger, Medical University, Poland.

Complete Peer review History: http://www.sciencedomain.org/review-history/24557

Abstracts

Incorporation of fruits and vegetables in diet can successfully be used in prevention and treatment of cancer. Annona muricata and Abelmoschus esculentus which belongs to the annonaceae and malvaceae family respectively have commonly been used in traditional medicine to treat various ailments. This study evaluated the phytochemical components of both A. muricata and A. esculentus and their antiproliferative activity on the breast, cervical and prostate cancer cell lines. Both A. muricata and A. esculentus were extracted using methanol and dichloromethane in a ratio of 1:1. Phytochemical screening was done using standard analytical procedures.  The MTT assay was used to evaluate the antiproliferative activity of A. muricata and A. esculentus extracts against breast cancer, cervical cancer, prostate cancer and Vero cell lines. Phytochemical screening confirmed that the fruit of A. muricata and the pods of A. esculentus are rich in saponins, tannins, alkaloids, terpernoids, glycosides, flavonoids and phenols. A. muricata had an IC50 of 23.632±1.3465 µg/ml, 72.5860±1.9819 µg/ml and 93.6233±3.0570 µg/ml on Hela (cervical cancer cells), DU145 (Prostate cancer) and HCC 1395 (Breast cancer) cells respectively. A. esculentus demonstrated antiproliferative activity on Hela cells with an IC50 of 20.3840±1.2132 µg/ml on DU145 and HCC 1395 cells an IC50 of 50.013±0.2502 µg/ml and 171.6460±4.7642 µg/ml respectively. The standard drug used had an IC50 of 21.126 µg/ml on HCC and 24.850 µg/ml on Hela cells. Both plants selectively inhibited the growth of the cancerous cells tested (SI>3) with the highest selectivity observed in HCC 1395 cells. This study authenticates traditional use and suggests potential use of these plants in cancer management and treatment.

Keywords :

Annona muricata; Abelmoschus esculentus; antiproliferative; phytochemical.

Full Article - PDF    Page 1-11

DOI : 10.9734/JOCAMR/2018/41248

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