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Journal of Advances in Medicine and Medical Research, ISSN: 2456-8899, ISSN: 2231-0614 (Past),Vol.: 26, Issue.: 6


Evaluation of Serum Copper (Cu), Serum Iron (Fe) and Serum Copper (Cu) / Iron (Fe) Ratio in Oral Submucous Fibrosis in Karachi


Haya Muhammad Khan1*

1Karachi Medical and Dental College, Ziauddin University, Karachi, Pakistan.

Article Information
(1) Babatunde Olanrewaju Motayo, Department of Pathology, Federal Medical Center, Abeokuta, Ogun State, Nigeria and Department of Virology, College of Medicine, University of Ibadan, Ibadan, Nigeria.
(2) Ibrahim ElSayed M. ElHakim, Professor, Ain Shams University, Egypt and Riyadh College of Dentistry and Pharmacy, Riyadh, Saudi Arabia.
(1) Sucheta Bansal, Himachal Pradesh University, India.
(2) Deepti Srivastava, I. T. S. Dental College, India.
(3) Saravanan Kandasamy, Vinayaka Missions Sankaracharya Dental College and Hospital, India.
Complete Peer review History:


Aims: To evaluate serum copper, serum iron, and copper/iron ratio in oral submucous fibrosis disease in Karachi.

Study Design: A cross-sectional study.

Place and Duration of Study: Ziauddin Hospital (Dental OPD), Karachi, Pakistan. In between the period of January 2014 to August 2015.

Methodology: This cross-sectional study included 130 oral submucous fibrosis patients, which were divided into 50 stage 1 OSMF, 50 stage 2 OSMF and 30 healthy individuals between the age range of 6-60 years (91 males and 37 females). Detailed questionnaire followed by sample collection of serum copper and iron from each patient was administered. Colorimetric assay analysed the samples.

Results: Mean age of OSMF patients was 34 years (15- 44 years). Most of them were males (70%), and less were females (30%). The highly affected group was of Muhajirs (Urdu speaking people) with the frequency of 40.8% as compared to other ethnicities. In the present study, the mean serum levels for copper (Cu) and iron (Fe) in healthy individuals were found to be 87 ± 20.2927 µg/dL and 73.7 ± 29.594 µg/dL respectively. In stage 1 OSMF patients, mean serum copper and iron levels were 149.56 µg/dL ± 10.8009 and 28.32 µg/dL ± 3.04 respectively. In stage 2 OSMF patients, mean serum copper and iron levels were 178.58 µg/dL ± 6.60887 and 18.82 µg/dL±2.21. Copper/ Iron ratio was also compared among different stages OSMF patients and healthy individuals. Among healthy individuals, copper/iron ratio was 1.2 which rose to 5.3 in OSMF stage 1, and the highest ratio was found in OSMF stage 2 with 9.5. It showed that the copper/iron ratio significantly increased with the severity of the disease (p < 0.0001).

Conclusion: Copper (Cu) and iron (Fe) could be used as biological markers in oral submucous fibrosis. Serum Cu to Fe ratio could be a helpful tool and a reliable biomarker in the diagnosis and prognosis of OSMF disease.

Keywords :

OSMF; serum; Cu; Fe; ratio.

Full Article - PDF    Page 1-9

DOI : 10.9734/JAMMR/2018/40845

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