Quick Menu

Upcoming Journals

Biotechnology Journal International

Biotechnology Journal International, 2456-7051,Vol.: 21, Issue.: 1

Original-research-article

Opuntia Extractable and Non-extractable (NEP) Residues as Co-carbon Sources for Improved Extracellular Laccase Production: Partial Purification and Characterization

 

Aruwa, Christiana E.1,2*

1Department of Microbiology and Biotechnology, Federal University of Technology Akure (FUTA), P.O.Box 704, Akure, Ondo State, Nigeria.

2Department of Biotechnology and Food Technology, Durban University of Technology (DUT), P.O.Box 1334, Durban 4000, South Africa.

 

Article Information
Editor(s):
(1) Anil Kumar, Professor, School of Biotechnology, Devi Ahilya University, India.
Reviewers:
(1) Olaolu Oyedeji, Obafemi Awolowo University, Nigeria.
(2) Devendra V. Deshmukh, ASC College Badnapur, Dr. Babasaheb Ambedkar Marathwada University, India.
(3) Alba Mónica Montiel González, Universidad Autónoma De Tlaxcala, México.
Complete Peer review History: http://www.sciencedomain.org/review-history/24158

 

Abstracts

 

Aim: The study of laccase production by Trametes pubescens cultured on cladode extractive residues alone, and as co-carbon source with wheat, using submerged fermentation condition.

Place and Duration of Study: Biotechnology Laboratory, Durban University of Technology, Durban, South Africa.

Methodology: Plant extraction, submerged fermentation, enzyme activity and characterization techniques were utilized.

Results: The highest laccase activity was observed at 60-80% wheat: residue ratio combinations under submerged conditions with copper and xylidine supplementation. Partially purified enzyme fractions showed similar characteristics at different temperatures and pHs. There was good retention of relative enzyme activity at temperatures near 60°C, and pH stability from pH 4.0-6.0. At optimal culture conditions laccase activity was highest at 49.01±0.21 U/ml (80% wheat: 20% residue ratio), and lower at 10.02±0.51 U/ml (80% wheat:20% NEP residue). The optimum temperature for laccase fractions was 25°C and pH optimum at 5. Highest specific activity was 3.55 U/mg of protein for the 80% wheat: 20% residue ratio laccase extract.

Conclusion: These results show the potential of cladode extractable phenol residues as potential economic growth medium for laccase production, offering a new alternative use for this agro-industrial and/or laboratory by-product.

 

Keywords :

Laccase; Trametes pubscens; cladode residues; NEP; wheat bran.

 

Full Article - PDF    Page 1-13    Article Metrics

 

DOI : 10.9734/BJI/2018/39647

Review History    Comments

Search this site

Advanced Search

Announcement & News

Nature (Impact Factor: 41.6) confirmed high standard of SDI journal and its editors

We are happy to inform that Nature (Impact Factor: 41.6) confirmed high standard of SDI journal and ...

ISI Thomson Reuters selected British Journal of Pharmaceutical Research for Emerging Sources Citation Index

We are delighted to inform that ISI Thomson Reuters selected British Journal of Pharmaceutical Resea...

SCOPUS selected Annual Research & Review in Biology (ARRB)

We are delighted to inform that famous indexing organization SCOPUS (from Elsevier) selected  A...

Index Copernicus Evaluation Result Released

We are delighted to inform that Index Copernicus (a leading indexing organization from Pol...

Journal Repository (JR): Permanent Digital Archiving of SDI journals

SDI is happy to announce that all our journals are now permanently archived in Journal Repository (J...

SDI journal got 35th ranking in Publons

We are delighted to announce (as of 04/01/2016) that British Journal of Medicine and Medical Re...

Growth of SDI and world publication market

As of 2014, total 25,064 journals are competing in World market of journal publication. In 2011, tot...

Science (IF: 31) report confirmed the high standard of SDI journal

As per a recent report (Link) of Science journal (present Impact factor 31), one of our journal (Bri...

SDI introduced Post-publication peer review by its comment section

SDI journals encourage Post-publication peer review by its comment section   Policy details a...

SDI promotes transparent Advanced OPEN peer review

We have migrated to transparent and toughest ‘Advanced OPEN peer review’ system (...



SCIENCEDOMAIN Awards

  • No Awards listed.

Browser Compatibility : Mozila firefox, Google Crome and IE 7 & above. Creative Commons License Terms & Condition   |   Privacy Policy   |   Join Us   |   Help   |   Contact Us
© Copyright 2010-2018, SCIENCEDOMAIN international. All rights reserved.