Biotechnology Journal International, 2456-7051,Vol.: 21, Issue.: 1
Opuntia Extractable and Non-extractable (NEP) Residues as Co-carbon Sources for Improved Extracellular Laccase Production: Partial Purification and Characterization
Aruwa, Christiana E.1,2* 1Department of Microbiology and Biotechnology, Federal University of Technology Akure (FUTA), P.O.Box 704, Akure, Ondo State, Nigeria. 2Department of Biotechnology and Food Technology, Durban University of Technology (DUT), P.O.Box 1334, Durban 4000, South Africa.
Aruwa, Christiana E.1,2*
1Department of Microbiology and Biotechnology, Federal University of Technology Akure (FUTA), P.O.Box 704, Akure, Ondo State, Nigeria.
2Department of Biotechnology and Food Technology, Durban University of Technology (DUT), P.O.Box 1334, Durban 4000, South Africa.
(1) Anil Kumar, Professor, School of Biotechnology, Devi Ahilya University, India.
(1) Olaolu Oyedeji, Obafemi Awolowo University, Nigeria.
(2) Devendra V. Deshmukh, ASC College Badnapur, Dr. Babasaheb Ambedkar Marathwada University, India.
(3) Alba Mónica Montiel González, Universidad Autónoma De Tlaxcala, México.
Complete Peer review History: http://www.sciencedomain.org/review-history/24158
Aim: The study of laccase production by Trametes pubescens cultured on cladode extractive residues alone, and as co-carbon source with wheat, using submerged fermentation condition.
Place and Duration of Study: Biotechnology Laboratory, Durban University of Technology, Durban, South Africa.
Methodology: Plant extraction, submerged fermentation, enzyme activity and characterization techniques were utilized.
Results: The highest laccase activity was observed at 60-80% wheat: residue ratio combinations under submerged conditions with copper and xylidine supplementation. Partially purified enzyme fractions showed similar characteristics at different temperatures and pHs. There was good retention of relative enzyme activity at temperatures near 60°C, and pH stability from pH 4.0-6.0. At optimal culture conditions laccase activity was highest at 49.01±0.21 U/ml (80% wheat: 20% residue ratio), and lower at 10.02±0.51 U/ml (80% wheat:20% NEP residue). The optimum temperature for laccase fractions was 25°C and pH optimum at 5. Highest specific activity was 3.55 U/mg of protein for the 80% wheat: 20% residue ratio laccase extract.
Conclusion: These results show the potential of cladode extractable phenol residues as potential economic growth medium for laccase production, offering a new alternative use for this agro-industrial and/or laboratory by-product.
Laccase; Trametes pubscens; cladode residues; NEP; wheat bran.
DOI : 10.9734/BJI/2018/39647Review History Comments
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