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Journal of Advances in Microbiology, 2456-7116,Vol.: 10, Issue.: 1

Original-research-article

Antimicrobial Resistance Profile and Molecular Characterization of Extended-spectrum Beta-lactamase Genes in Enterobacteria Isolated from Human, Animal and Environment

 

Gbonon M’bengue Valérie Carole1*, Guessennd Nathalie Kouadio1,2, Ouattara Mohamed Baguy1,3, Ouattara Gnoh Djénéba1, Abraham Ayayi4,  Tiekoura Konan Bertin1, Toty Abalé Anatole1, Kouadio Kouamé Innocent1,3, Dissinviel Stéphane Kpoda5, Tahou Eric1, Konate Ali6, Konan Fernique1, Kamenan Alphonse3, Dosso Mireille1 and Ger Bmr1

1Department of Bacteriology-Virology, National Reference Center for Antibiotics, Pasteur Institute of Côte d'Ivoire, Abidjan, Côte d'Ivoire.

2Laboratory of Bacteriology-Virology, Department of Medical Sciences, Félix Houphouet - Boigny University, Abidjan, Côte d'Ivoire.

3Laboratory of Microbiology and Molecular Biology, Department of Food Science and Technology, Nangui Abrogoua University, Abidjan, Côte d'Ivoire.

4Department of Microbiology, University of Lagos, Nigeria.

5Laboratory of Applied and Nutritional Sciences, Ouaga I Professor Joseph KI-ZERBO University, Ouagadougou, Burkina Faso.

6Laboratory of Molecular Biology, Epidemiology and Monitoring of Foodborne Bacteria and Viruses, Ouaga I Professor Joseph KI-ZERBO University, Ouagadougou, Burkina Faso.

Article Information

Editor(s):

(1) Adekunle Sanyaolu, Epidemiology Division, Nigeria Center for Disease Control, Federal Ministry of Health, Abuja, Nigeria.

Reviewers:

(1) Ruan Carlos Gomes da Silva, Centro Universitário Tabosa de Almeida, Brazil.

(2) Iroha Ifeanyichukwu Romanus, Ebonyi State University, Nigeria.

Complete Peer review History: http://www.sciencedomain.org/review-history/24131

Abstracts

Objective: The aim of this study is to determine the antibiotic resistance profile and characterize extended spectrum beta-lactamase gene of enterobacteria strains isolated from human biological products, fecal matter of animals and the environment.

Materials and Methods: Enterobacteria producing ESBL strains were isolated from human products, fecal matter of healthy animals (cattle, sheep and pigs) intended for human consumption and environment (hospital effluents and municipal sewage) using homemade medium (Drigalski supplemented with 2 mg/L of ceftazidime). Resistance to beta-lactams has been evaluated by the diffusion method was carried out as recommended by NCCLS. Characterization of Beta-Lactamase resistance genes (blaCTXM, blaSHV, blaTEM, blaGES, blaPER and blaVEB) was performed by simplex and multiplex PCR.

Results: The strains were resistant to antibiotics from beta-lactam family (penicillin with inhibitor, monobactam, cephalosporin) but no resistant was observed to carbapenem (imipénème, méropénème). All resistance genes were identified in environment strains.

Conclusion: This study showed the presence of common beta-lactam resistance genes (blaTEM, blaSHV and blaCTX-M) to human, animal and environment. The risk of dissemination and circulation of ESBL enterobacteria between animals, humans and the environment exists in Ivory Coast because of the absence of a barrier between them.

Keywords :

Enterobacteria ESBL; human; animal; environment; Ivory Coast.

Full Article - PDF    Page 1-9

DOI : 10.9734/JAMB/2018/39955

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