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Journal of Pharmaceutical Research International, ISSN: 2456-9119, ISSN: 2231-2919 (past),Vol.: 21, Issue.: 3


Comparison of the Efficacy of Different Hydrocortisone-Induced Cataract Models in Developing Chick Embryos


Reşat Duman1*, Tolga Ertekin2, Rahmi Duman1, Ayhan Vurmaz3, Tülay Köken3  and Abdülkadir Bilir2

1Department of Ophthalmology, School of Medicine, Afyon Kocatepe University, Afyonkarahisar, Turkey.

2Department of Anatomy, School of Medicine, Afyon Kocatepe University, Afyonkarahisar, Turkey.

3Department of Clinical Biochemistry, School of Medicine, Afyon Kocatepe University, Afyonkarahisar, Turkey.

Article Information
(1) Vijay Ramani, College of Pharmacy, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA.
(1) Asaad Ahmed Ghanem, Mansoura University, Egypt.
(2) Italo Giuffre, Catholic University of Roma, Italy.
Complete Peer review History: http://www.sciencedomain.org/review-history/23500


Aim: To evaluate and compare the efficacy of different hydrocortisone-induced cataract models in chick embryos.

Material and methods: On the 15th day of the study, hydrocortisone succinate sodium (HC) (0.5 μmol/egg or 0.25 μmol/egg) was administered directly into the air sac (AS) or into the chorioallantoic membrane (CAM) in 4 HC-groups. In two control groups, phosphate-buffered saline (PBS) was administered in the same manner. On the 17th day of the study (48 hours after the injection), the lenses were removed and classified into five stages according to lens opacification under a stereoscopic microscope. In addition, reduced glutathione levels (GSH) of the lenses were measured by High-performance liquid chromatography (HPLC) method.

Results: The mean scores of lens opacity and cataract formation in HC-groups were significantly higher than those of control groups (p = 0.00). In addition, the mean reduced glutathione levels were significantly lower in HC-groups compared to control groups (199-245 ps vs. 291-294, p = 0.00). The mean scores for lens opacity, cataract formation, and the mean GSH levels did not significantly differ among 4 HC-groups (p > 0.05).

Conclusion: Both doses of HC (0.25 or 0.50 µmol/egg) and both injection methods (into the AS or CAM) may be used efficiently for cataract formation in chick embryo steroid-induced cataract models.

Keywords :

Cataract; eye; hydrocortisone; chick.

Full Article - PDF    Page 1-7

DOI : 10.9734/JPRI/2018/39888

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