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European Journal of Medicinal Plants, ISSN: 2231-0894,Vol.: 22, Issue.: 3


In-vitro Assessment of Cholinesterase Inhibitory and Thrombolytic Activity of Six Available Citrus Fruits in Bangladesh: Relevant for Treating Neurodegenerative Disorder


Kushal Biswas1*, Saiyara Hossain1, Sanzida Alam1, Taposhi Sultana1, Farzana Khan1 and Sanjay Dutta2

1Department of Pharmacy, East West University, Bangladesh.

2Department of Pharmacy, Khwaja Yunus Ali University, Bangladesh.

Article Information


(1) Shanfa Lu, Professor, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, China.

(2) Marcello Iriti, Professor, Plant Biology and Pathology, Department of Agricultural and Environmental Sciences, Milan State University, Italy.


(1) Muhammad Ali, Kano University of Science and Technology, Nigeria.

(2) Yongchun Zhu, Shenyang Normal University, China.

(3) Francisco José Queiroz Monte, Universidade Federal do Ceará, Brasil.

Complete Peer review History: http://www.sciencedomain.org/review-history/23447


Aim: Citrus fruits are well known for its medicinal and food value. Aim of this study is to investigate acetylcholinesterase ((AChE)) inhibitory activity, butyrylcholinesterase (BuChE) inhibitory activity, total phenolics, flavonoids, flavonols content and thrombolytic activities of crude methanol extracts of 6 citrus fruits (Citrus limon, Citrus aurantifollia, Citrus bergamia, Citrus maxima, Citrus sinensis and Citrus macroptera).

Methods: The fruits were extracted by using methanol as solvent. Ellman’s colourimetric method was applied to determine both cholinesterase inhibitory activities, while folin-ciocalteau reagent (FCR) and aluminium chloride were used to quantify total phenolics, flavonoids, flavonol content of those fruits. Blood clot lysis method was applied for determining the thrombolytic activity of those fruits.

Results: All citrus fruits contain a good amount of phenolics, flavonoids and flavonols. C. maxima found more prominent in containing phenolics and flavonols compare to other citrus fruits, with 414.06 ± 2.87 mg Gallic Acid Equivalent/gm and 12.94 ± 1.31 mg Catechin Equivalent/gm dried extract respectively. Citrus sinensis showed the highest content in flavonoids with 21.16± 1.37 mg Catechin 20 Equivalent /gm dried extract. Citrus fruits are also a quality source of cholinesterase inhibitors. All the examined citrus fruits were found capable of inhibiting both acetylcholinesterases (AChE) as well as butyrylcholinesterase (BuChE). C. bergamia was most effective in inhibiting AChE with IC50 of 27.18 µg/ml where C. macroptera was best in inhibiting BuChE (IC50 32.5 µg/ml). But none of the citrus fruits was found fit for thrombolytic activity.

Conclusion: Citrus fruits are found the sound in inhibiting AChE and BuChE as well as containing Phenolics, flavonoids and flavonols. But they lack in their thrombolytic activity.

Keywords :

Citrus fruit; phenolics; flavonoids; flavonols; cholinesterase inhibition.

Full Article - PDF    Page 1-11

DOI : 10.9734/EJMP/2018/39693

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