Asian Journal of Advances in Agricultural Research, 2456-8864,Vol.: 5, Issue.: 1
Citric Acid Production by Aspergillus niger and Trichoderma viride Using Hydrolysed Potato Peels Substrate
M. D. Makut1 and I. K. Ekeleme1* 1Department of Microbiology, Nasarawa State University, Keffi, Nasarawa State, Nigeria.
M. D. Makut1 and I. K. Ekeleme1*
1Department of Microbiology, Nasarawa State University, Keffi, Nasarawa State, Nigeria.
(1) Tancredo Souza, Department of Life Sciences, Centre for Functional Ecology, University of Coimbra, Portugal.
(1) Elias Ernesto Aguirre Siancas, Scientific University of the South, Peru.
(2) Selma Gomes Ferreira Leite, Federal University of Rio de Janeiro, Brasil.
(3) Reinaldo Gaspar Bastos, Federal University of São Carlos, Brazil.
(4) R. Prabha, Animal and Fisheries Sciences University, India.
Complete Peer review History: http://www.sciencedomain.org/review-history/23156
Aim: Production of citric acid by Aspergillus niger and Trichoderma viride isolated from soil in Keffi, Nigeria using Hydrolysed potato peels substrate.
Place and Duration of the Study: This study was carried out in the Department of Microbiology Faculty of Natural and Applied Sciences Nassarawa State University Keffi, Nigeria, between February and July 2017.
Methodology: Aspergillus niger and Trichoderma viride were isolated from soil in Keffi and identified using standard microbiology methods. Hydrolysed potato peels substrate - soy beans cake (ps) and potato peel - groundnut cake (pg) production media were prepared by following standard fermentation conditions. The citric acid produced was estimated using Gas Chromatography/Mass Spectrometry (GC/MS) method respectively.
Results: At pH 5.5 after 72 hours of incubation the highest production of citric acid was produced by T. viride 176.33±20.10 mg/l on ps and A. niger 91.11±21.22 mg/l on ps. At pH 6.0 T. viride produced highest on ps 211.40±7.10 mg/l and A. niger 170.22±10.81 mg/l on ps while at pH 6.5 A. niger produced highest 181.09±20.8 mg/l on ps and T. viride 98.76±19.8 mg/l on ps with highest total Sugar consumption and dry cell mass were 16.19±20.01 g/l and 13.17±1.04 g/l while after 144 hours of incubation at pH 6.0 A. niger produced highest citric acid 404.53 ± 14.32 mg/l on ps and T. viride produced 297.60±8.31mg/l on ps highest total Sugar consumption and dry cell mass were 19.91±2.01g/l and 17.0±15.11 g/l.
Conclusion: The maximum citric acid production obtained during the course of study was 404.53±14.32 mg/l on ps by A. niger and at pH 6.0. Also potato peels showed that it can be alternative carbon source for citric acid production as cheaper source of carbon.
Aspergillus niger; Trichoderma viride; ps and pg substrate.
DOI : 10.9734/AJAAR/2018/38268Review History Comments