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Annual Research & Review in Biology

Annual Research & Review in Biology, ISSN: 2347-565X,Vol.: 24, Issue.: 1

 

Original-research-article

 

Activators and Inhibitors of α-glucosidase from Penicillium chrysogenum

 

 

Hamed M. El-Shora1*, Mohsen E. Ibrahim2 and Mohammad W. Alfakharany2

1Department of Botany, Faculty of Science, Mansoura University, Dakahlia, Egypt.

2Department of Botany, Faculty of Science, Port Said University, Port Said, Egypt.

 

Abstracts

 

α-glucosidase (EC: 3.2.1.20) from Penicillium chrysogenum Thom ATCC 10106 was induced by GSH at the lower concentrations. H2O2 was inhibitor at all tested concentrations and the IC50 was 92.2%v/v. AMP, ADP and ATP enhanced the activity revealing that α-glucosidase is endothermic enzyme. The chelating agents are ethylenediaminetetraacetate (EDTA), α-α-dipyridyl and o-phenanthroline inhibited the enzyme. IC50 for these three compounds were 7.1, 10.2 and 10.9 mM, respectively. The highest activity of α-glucosidase was recorded at 150 mM phosphate buffer. Mannitol as polyol protected the enzyme against heat inactivation. The five sugars trehalose, lactose, raffinose, glucose and sucrose protected α-glucosidase against thermo-inactivation at 60ºC. Also, sarcosine as a product of glycine provided α-glucosidase with appreciable thermostability at 60ºC.

 

Keywords :

P. chrysogenum; glutathione; adenosine compounds; chelating agents; trehalose; mannitol. 

 

Full Article - PDF    Page 1-9    Article Metrics

 

DOI : 10.9734/ARRB/2018/38408

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