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Microbiology Research Journal International, 2456-7043,Vol.: 22, Issue.: 4

Original-research-article

Anti-Coccidiosis Potential of Autoclaveable Antimicrobial Peptides from Xenorhabdus budapestensis Resistant to Proteolytic (Pepsin, Trypsin) Digestion Based on In vitro Studies

 

András Fodor1*, László Makrai2, László Fodor2, István Venekei3, Ferenc Husvéth4, László Pál4, Andor Molnár4, Károly Dublecz4, Csaba Pintér5, Sándor Józsa5 and Michael G. Klein6

1Department of Genetics, Faculty of Science and Informatics, University of Szeged, Közép fasor 52, Szeged, H-6726, Hungary.

2Department of Microbiology and Infectious Diseases, University of Veterinary Medicine, Budapest, Hungary.

3Department of Biochemistry, Eötvös Loránd University, Budapest H-1117, Hungary.

4Department of Animal Sciences and Animal Husbandry, Georgikon Faculty, University of Pannonia, Széchenyi Street, 11 Keszthely, H-8360 Hungary.

5Georgikon Faculty, University of Pannonia, Deák Ferenc utca, 16, Keszthely, H-8360, Hungary.

6Department of Entomology, The Ohio State University, 1680 Madison Ave., Wooster, OH-44691, USA.

Article Information
Editor(s):
(1) Xing Li, Division of Biomedical Statistics and Informatics, Department of Health Sciences Research, Mayo Clinic College of Medicine, USA.
Reviewers:
(1) Luz del Carmen Camacho Castillo, Instituto Nacional de Pediatría, México.
(2) Nagaraja Suryadevara, MAHSA University, Malaysia.
Complete Peer review History: http://www.sciencedomain.org/review-history/22705

Abstracts

Aims: To elucidate the anticoccidial potential of antimicrobial peptides from Xenorhabdus budapestensis on both causative pathogens (prokaryotic Clostridium perfringens and eukaryotic Eimeria tenella).

Objectives: (1) To establish if the antimicrobial compounds of the cell-free culture media (CFCM) of the entomopathogenic symbiotic bacterium species, X. budapestensis DSM 16342 (EMA) and X. szentirmaii DSM 16338 (EMC) were active against 13 independent pathogenic isolates of Clostridium perfringens in vitro; (2) To create a sterile, autoclaved, bio-preparation called “XENOFOOD”, for future in vivo feeding studies, aimed at determining the efficacy, and side-effects, of EMA and EMC on C. perfringens in chickens.

Study Design: Clostridium perfringens samples (LH-1-LH24) were collected from chickens and turkeys, and were deposited in the frozen stock collection of Department of Microbiology and Infectious Diseases, Faculty of Veterinary Science, Szent István University, Budapest, Hungary, where the in vitro assays were carried out on 13 of these isolates.

Place and Duration of Study: Department of Microbiology and Infectious Diseases, Faculty of Veterinary Science, Szent István University, Budapest, Hungary between September 2013 and February 2014.

Methodology: Adaptation of our previously published in vitro bioassays for aerobic tests for the anaerobic bacteria Clostridium perfringens. When preparing “XENOFOOD” we benefitted from our experimental data about the heat tolerance and endurance to proteolytic enzymatic digestion of the studied antimicrobial peptides.

Results: The studied antimicrobial peptides were heat-stable, trypsin and pepsin resistant. All but one of 13 C. perfringens isolates was sensitive to EMA-CFCM. XENOFOOD (made here) is not toxic for chicken, (unpublished).

Conclusion: Since these cell-free cultures killed E. tenella cells, but were toxic to permanent chicken liver (LMH) cells, we need to run in vivo feeding tests to determine the gastrointestinal (ileac), anti-Clostridium and anti-Eimeria biological effects of the these heat, - and proteolysis tolerant antimicrobial peptides.

Keywords :

Clostridium perfringens; Xenorhabdus antimicrobial peptides; in-vitro bioassay; XENOFOOD.

Full Article - PDF    Page 1-17

DOI : 10.9734/MRJI/2017/38516

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