Advertisement
+91 8617752708

Journal of Advances in Medicine and Medical Research, ISSN: 2456-8899, ISSN: 2231-0614 (Past),Vol.: 25, Issue.: 1

Original-research-article

Chlamydia Infection-derived Exosomes Possess Immunomodulatory Properties Capable of Stimulating Dendritic Cell Maturation

 

Raedeen S. Russell1, Khamia Ryans2, Ming Bo Huang1, Yusuf Omosun1, Mahfuz Khan1, Michael D. Powell1, Joseph U. Igietseme3 and Francis O. Eko1*

1Department of Microbiology, Biochemistry and Immunology, Morehouse School of Medicine, Atlanta, GA, USA.

2Department of Biology, Clark Atlanta University, Atlanta, GA, USA.

3National Center for Emerging Zoonotic and Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA.

Article Information

Editor(s):

(1) E. Umit Bagriacik, Department of Immunology, Gazi University, Turkey.

Reviewers:

(1) Alcibey Alvarado, Costa Rica.

(2) Chia-Li Yu, National Taiwan University Hospital, Taiwan.

(3) Seiichi Kitagawa, Osaka-City University, Japan.

Complete Peer review History: http://www.sciencedomain.org/review-history/22687

Abstracts

Aims: The aim of this study was to characterize exosomes derived from Chlamydia muridarum-infected and normal healthy untreated mouse oviduct epithelial cells (MOEC) and evaluate their immunomodulatory properties.

Methodology: Exosomes were purified from C. muridarum-infected and uninfected MOEC and analyzed using the NanoSight nanoparticle tracking analysis system. The concentration of cytokines and chemokines associated with exosomes and secreted by exosome-activated bone marrow-derived dendritic cells (BMDCs) was assessed using the Bio-Plex cytokine assay kit in combination with the Bio-Plex Manager software. Also, the exosome-stimulated dendritic cell expression of co-stimulatory surface and major histocompatibility complex class II (MHC-II) molecules was assessed by FACS analysis.

Results: The results showed that the concentration of cytokines (IL-6, IL-10, IL-12, IFN-γ and TNF-α) and chemokines (KC (CXCL1), MCP-1 (CCL2), MIP-1α (CCL3), Rantes (CCL5) and Eotaxin (CCL11)) secreted by BMDCs pulsed with Chlamydia infection-derived exosomes (IDEX) was significantly higher (p < 0.05) than that secreted by BMDCs pulsed with uninfected control-derived exosomes (CDEX). Furthermore, exosomes purified from Chlamydia-infected MOEC significantly upregulated (p < 0.05) the dendritic cell expression of CD86 and MHC-II, molecules associated with DC activation and maturation, compared to those from uninfected cells.

Conclusion: The results indicate that Chlamydia IDEX possess immunomodulatory properties capable of stimulating dendritic cell activation and maturation. Further studies will delineate their potential use as immunomodulators or as vaccine delivery vehicles.

Keywords :

Exosomes; Chlamydia; infection; proinflammatory cytokines; immunomodulation.

Full Article - PDF    Page 1-15

DOI : 10.9734/JAMMR/2018/38821

Review History    Comments

Our Contacts

Guest House Road, Street no - 1/6,
Hooghly, West Bengal,
India

+91 8617752708

 

Third Floor, 207 Regent Street
London, W1B 3HH,
UK

+44 20-3031-1429