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British Microbiology Research Journal, ISSN: 2231-0886,Vol.: 4, Issue.: 1 (January)

Original-research-article

Molecular Detection of Antimicrobial Resistance Gene Cassettes Associated with Class 2 Integron in Salmonella Serovars Isolated in Iran

 

Bahareh Rajaei1,2, Seyed Davar Siadat1,7*, Nahid Sepehri Rad1, Farzad Badmasti1, Mohamad Reza Razavi3, Mohammad Reza Aghasadeghi4, Raheleh Saboohi1, Taraneh Rajaei5, Arfa Moshiri1,6, Mehdi Nejati1 and Ahmad Reza Bahremand7

1Department of Microbiology, Pasteur Institute of Iran, Tehran, Iran.
2Department of Stem Cells, Division of Nanobiomaterials and Tissue Engineering, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran.
3Department of Parasitology, Pasteur Institute of Iran, Tehran, Iran.
4Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran.
5Department of biotechnology, Pasteur Institute of Iran, Tehran, Iran.
6Department of Biotechnology, School of Allied Sciences, Tehran University of Medical Sciences, Tehran, Iran.
7Department of Mycobacteriology and Lung Research, Pasteur Institute of Iran, Tehran, Iran.

Article Information

Editor(s):

(1) Giuseppe Blaiotta, Department of Food Science, Via Università, Italy.

Reviewers:

(1) Anonymous.

(2) Anonymous.

(3) G. Kaushik Subramanian, University of Madras, India.

(4) Anonymous.

Complete Peer review History:http://www.sciencedomain.org/review-history/2267

Abstracts

Aim:Salmonella is an important food-borne pathogen in humans and a broad range of animals. Antimicrobial resistance in Salmonella spp. is a serious health problem in human and veterinary medicine worldwide. The aim of this study was to detect integrons, the natural recombination systems that can be transferred in companion with mobile genetic elements and play a major role in spreading antibiotic resistance genes in clinical isolates.
Place and Duration of Study:Salmonella clinical isolates were provided by a number of institutes and hospitals over the country through the years 2008-2009.
Methodology: Antimicrobial susceptibility testing and serotyping were carried out for eighty four epidemiologically unrelated clinical isolates of Salmonella serovars collected from different provinces of Iran through the years 2008-2009. PCR assays were carried out to detect intI2 gene (integrase I attributed to class 2 integron) and internal variable regions (IVRs) of class 2 integron. These sequences were deposited in EMBL/GenBank database (www.ncbi.nlm.nih.gov).
Results: Eleven isolates (13.1%) which were resistant to at least 4 groups of antimicrobial agents were considered as MDR (multidrug resistant) Salmonella serovars. PCR assays detected intI2 gene (integrase I attributed to class 2 integron) and internal variable regions (IVRs) of class 2 integron in Fourteen (16.7%) and eleven (78.6%) of Salmonella clinical isolates respectively. Analysis of the sequence data revealed 3 gene cassette arrays deposited in Genbank databases including the dhfrA1 (0.75 kb), dfrA14- lsp (1 kb), dhfrA1-sat2-aadA1 (3 kb) with three IVR distribution patterns. An artifact PCR product of 2 kb was reported in this study to be amplified together with IVRs of class 2 integrons which was associated with the fhuE- ptsG genes.
Conclusions: Presence of MDR Salmonella serovars demonstrates that antimicrobial selection pressure is widespread in our clinical settings. Detection of class 2 integron carrying gene cassettes which confer resistance to different classes of antibiotics such as aminoglycosides, and trimethoprim confirms that integron-mediated antimicrobial gene cassettes are prevalent in Salmonella serovars isolated in Iran.

Keywords :

Salmonella; class 2 integron; gene cassettes; multidrug resistance (MDR).

Full Article - PDF    Page 132-141 Article Metrics

DOI : 10.9734/BMRJ/2014/4639

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