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Annual Research & Review in Biology, ISSN: 2347-565X,Vol.: 21, Issue.: 5


Studies on Establishment of Aseptic Culture in Pomegranate cv. Bhagwa


Prabhuling Guranna1* and Huchesh C. Hoolageri1

1Centre for Horticulture Biotechnology, Directorate of Research, University of Horticulture Sciences, Bagalkot 587104, Karnataka State, India.

Article Information
(1) George Perry, Dean and Professor of Biology, University of Texas at San Antonio, USA.
(1) Manoj Kumar Yadav, Sardar Vallabhbhai Patel University of Agriculture and Technology, India.
(2) Carla Benelli, Italy.
Complete Peer review History: http://www.sciencedomain.org/review-history/22531


Pomegranate (Punica granatum L.) is an economically important fruit crop of arid and semiarid regions of the world. Micropropagation of pomegranate, have provided many advantages over the conventional methods of vegetative propagation. It ensures true to type of plants, uniform quality and rapid mass production of disease free planting materials with seasonal independency. The present study was carried out with the objective to standardize efficient and reproducible protocol for establishment of aseptic culture in pomegranate “Bhagwa”. Different explants, duration of mercuric chloride treatment and antioxidants were tried for culture initiation. Among the various explants, maximum aseptic culture (55.00%) establishment was obtained with double nodal explants containing third + fourth nodes. The effective surface sterilization with lowest contamination (15%) was achieved with HgCl2 (0.10%) for 3 minute. Lesser intensity of browning and higher culture establishment (54%) was observed with 2000 mg/l activated charcoal.

Keywords :

Aseptic culture; double nodal segment; axillary bud; browning; Bhagwa.

Full Article - PDF    Page 1-7

DOI : 10.9734/ARRB/2017/38807

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