Quick Menu

Upcoming Journals

Journal of Experimental Agriculture International

Journal of Experimental Agriculture International, ISSN: 2457-0591, ISSN: 2231-0606 (Past),Vol.: 19, Issue.: 4


Proteomic Analysis of Natural and Demucilaged Coffee Beans from Plantations at Different Altitudes in the Mantiqueira Mountains


Kalynka G. do Livramento1*, Flávio M. Borém1,2, Luana F. Torres1, Fabrício L. da Silva1, Dárlan Einstein do Livramento3 and Luciano V. Paiva1,4

1Federal University of Lavras (UFLA), Lavras, Minas Gerais, Brazil.

2Department of Engineering, Federal University of Lavras (UFLA), Brazil.

3State University of Passos (UEMG), Passos, Minas Gerais, Brazil.

4Department of Chemistry, Federal University of Lavras (UFLA), Brazil.


Article Information
(1) Marco Aurelio Cristancho, Professor, National Center for Coffee Research, CENICAFÉ, Colombia.
(1) Adden Ayi Koffi, Togo.
(2) Chunlong li, Cornell Universuty, USA.
(3) Javan Ngeywo, Kenya.
(4) Fatema Hossain Brishti, Universiti Putra Malaysia, Malaysia.
(5) Widi Sunaryo, Mulawarman University, Indonesia.
Complete Peer review History: http://www.sciencedomain.org/review-history/22526




Analysis of the effects of climatic conditions and processing methods on coffee beans is of great interest in research, chemical composition and consequent beverage quality are strongly influenced by growing conditions and/or processing methods.

Aims: In this study, beans of Coffea arabica cv. Acaiá coffee trees grown at two altitudes (below 1000 m and above 1200 m) were assessed through a proteomic approach in an attempt to better understand the protein changes that occur during two types of processing (dry and wet processing).

Methodology: Samples of proteins of natural and demucilaged coffee bean were analyzed by two-dimensional electrophoresis. The gels stained with Commassie Blue G250 were evaluated by the ImageMaster 2D Platinum 7.0 program. The differentially abundant proteins were treated with the enzyme Trypsin e aliquots of each hydrolysate sample were analyzed by mass spectrometry. Sample molecular mass profile obtained through MS e MS/MS was subjected to comparative analysis using the MASCOT program, Brazilian Coffee Genome database, NCBI and Coffee Genome Hub.

Place and Duration of Study: The experiments were conducted in the following laboratories: the Laboratório Central de Biologia Molecular (LCBM) and the laboratório de Processamento de Produtos Agricolas (LPPA) at Federal University of Lavras, (Brazil),  between 2014 and 2017.

Results: Differentially accumulated proteins, such as 11S globulin, glyceraldehyde-3-phosphate dehydrogenase, dehydrin, and Heat shock proteins, have been found among treatments through mass spectrometry.

Conclusion: These results contribute to an understanding of the effects of altitude and processing methods on the chemical composition of coffee beans, and thus serve as a basis for future research related to coffee beverage quality.


Keywords :

2-DE; coffee protein content; coffee beverage quality; coffee processing; effects of altitude; protein analysis; proteome.


Full Article - PDF    Page 1-15    Article Metrics


DOI : 10.9734/JEAI/2017/38343

Review History    Comments

Search this site

Advanced Search

Announcement & News

Nature (Impact Factor: 41.6) confirmed high standard of SDI journal and its editors

We are happy to inform that Nature (Impact Factor: 41.6) confirmed high standard of SDI journal and ...

ISI Thomson Reuters selected British Journal of Pharmaceutical Research for Emerging Sources Citation Index

We are delighted to inform that ISI Thomson Reuters selected British Journal of Pharmaceutical Resea...

SCOPUS selected Annual Research & Review in Biology (ARRB)

We are delighted to inform that famous indexing organization SCOPUS (from Elsevier) selected  A...

Index Copernicus Evaluation Result Released

We are delighted to inform that Index Copernicus (a leading indexing organization from Pol...

Journal Repository (JR): Permanent Digital Archiving of SDI journals

SDI is happy to announce that all our journals are now permanently archived in Journal Repository (J...

SDI journal got 35th ranking in Publons

We are delighted to announce (as of 04/01/2016) that British Journal of Medicine and Medical Re...

Growth of SDI and world publication market

As of 2014, total 25,064 journals are competing in World market of journal publication. In 2011, tot...

Science (IF: 31) report confirmed the high standard of SDI journal

As per a recent report (Link) of Science journal (present Impact factor 31), one of our journal (Bri...

SDI introduced Post-publication peer review by its comment section

SDI journals encourage Post-publication peer review by its comment section   Policy details a...

SDI promotes transparent Advanced OPEN peer review

We have migrated to transparent and toughest ‘Advanced OPEN peer review’ system (...


  • No Awards listed.

Browser Compatibility : Mozila firefox, Google Crome and IE 7 & above. Creative Commons License Terms & Condition   |   Privacy Policy   |   Join Us   |   Help   |   Contact Us
© Copyright 2010-2018, SCIENCEDOMAIN international. All rights reserved.