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Journal of Advances in Biology & Biotechnology, 2394-1081,Vol.: 16, Issue.: 3

Original-research-article

In vitro Rapid Regeneration of Betel Vine (Piper betle L.)

 

Md. Mahabub Elahi1, Homayra Huq1, M. E. Hoque1 and Fahima Khatun1*

1Department of Biotechnology, Sher-e-Bangla Agricultural University, Bangladesh.

Article Information

Editor(s):

(1) Mohamed Ali Abdel-Rahman, Associate Professor, Department of Botany and Microbiology, Faculty of Science (Boys Branch), Al-Azhar University, Cairo, Egypt.

Reviewers:

(1) Aneta Gerszberg, University of Lodz, Poland.

(2) Jigna G. Tank, Saurashtra University, India.

Complete Peer review History: http://www.sciencedomain.org/review-history/22502

Abstracts

The experiment was conducted on betel vine (Piper betle L.) for in vitro regeneration in Biotechnology Lab. of the Department of Biotechnology, Sher-e-Bangla Agricultural University, Bangladesh from the period of July 2014 to April 2015 to investigate the effect of BA, KIN, IAA and IBA in MS medium, where nodal segment was used as explants. The highest percent of shoot proliferation (70%) was achieved with 1.5 mg/L BA + 3.0 mg/L KIN whereas the highest number of shoots and leaves were obtained in 1.0 mg/L BA + 3.0 mg/L KIN. The maximum 14.40 roots/explants were observed with 3.0 mg/L IBA compare with IAA. For hardening the regenerated plantlets were transferred to growth chamber where maximum survival rate (90%) was observed whereas 75% plantlets were survived in shade house in soil which was higher than in open atmosphere (50%).This experiment can be used as a reliable protocol for in vitro regeneration of betel vine.

Keywords :

In vitro; rapid regeneration; BA; KIN; IAA; IBA.

Full Article - PDF    Page 1-11

DOI : 10.9734/JABB/2017/38630

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