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Journal of Advances in Biology & Biotechnology, 2394-1081,Vol.: 16, Issue.: 3

Original-research-article

Phenotypic Evaluation of Cassava (Manihot esculenta Crantz) Genotypes to Cassava Mosaic Virus by Mechanical Methods of Transmission

 

Jerome Anani Houngue1*, Gilles Habib Todjro Cacaï1, Justin S. Pita2, Martine Zandjanakou -Tachin3, Edwige Fougnigué Yeo2 and Corneille Ahanhanzo1

1Central Laboratory of Plant Biotechnology and Plant Breeding, Department of Genetic and Biotechnology, Faculty of Sciences and Techniques, University of Abomey-Calavi, Benin.

2Laboratory of Plant Physiology, Université Félix Houphouët-Boigny, Côte d’Ivoire.

3Laboratory of Molecular Plant Pathology, School of Horticulture and Green Space, University of Agriculture of Kétou, Benin.

Article Information

Editor(s):

(1) Andrzej Kowalski, Department of Biochemistry and Genetics, Institute of Biology, Jan Kochanowski University, Kielce, Poland.

Reviewers:

(1) M. Ali Sevik, University of Ondokuz Mayis, Turkey.

(2) Kathleen Hefferon, Cornell University, USA.

Complete Peer review History: http://www.sciencedomain.org/review-history/22428

Abstracts

Aims: Cassava (Manihot esculenta Crantz), subsistence crop in sub-Saharan Africa was threatened by cassava mosaic virus that caused a devastated disease. This study aims to test thermotherapy as sanitation method in mechanical transmission study of cassava mosaic virus.

Methodology: Cuttings of different cultivars were treated by heating during two hours and twenty four hours at 50°C before potting. Four mechanical techniques of virus transmission were used. Contact technique consisting to put an infected plant with a healthy plant together by one junction point and the graft technique involved the grafting of axillary buds of diseased plants to healthy plants. Latex technique consisted of passing the latex from the infected plants on the injured healthy plants several times and sap technique involved the injection of inoculum prepared from the infected leaves to the healthy plants.

Results: Four cultivars had a sprouted rate superior to 80% by two hours of heating and less than 50% by 24 hours of heating. The bands related to ACMV or EACMV were not observed in the samples of different cultivars. Seventy five per cent (75%) of plants inoculated by contact technique had a perfect adhesion with 65% of plants expressing the mosaic symptoms. Six plants out of fourteen (6/14) of the plants of Hombete inoculated with latex expressed the disease symptoms and three plants out of ten (3/10) of the plants of Ornania expressed the disease symptoms. Hundred per cent (100%) of the plants of Djadjakor inoculated by grafting expressed the disease symptoms. Any plants of Atinwewe and Adjatidaho inoculated by Sap technique did not express the disease symptoms.

Conclusion: These results suggest that heat is effective for virus elimination and grafting constitutes the mechanical transmission technique which can be used to screen cassava germplasm for virus resistance.

Keywords :

Manihot esculenta; mechanical transmission; virus; inoculation; thermotherapy.

Full Article - PDF    Page 1-9

DOI : 10.9734/JABB/2017/38149

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