Quick Menu

Upcoming Journals

Asian Journal of Biotechnology and Genetic Engineering

Asian Journal of Biotechnology and Genetic Engineering, XXXX,Vol.: 1, Issue.: 1


Metagenomic Assessment of Antibiotics Resistance Genes from Four Ecosystems in the Niger Delta Area of Nigeria


U. O. Edet1,2*, S. P. Antai2, A. A. Brooks2 and A. D. Asitok2

1Department of Microbiology, Faculty of Natural and Applied Sciences, Obong University, Obong Ntak, Etim Ekpo LGA, Akwa Ibom State, Nigeria.

2Department of Microbiology, Faculty of Biological Sciences, University of Calabar, Calabar, Cross River State, Nigeria.


Article Information
(1) Tsygankova Victoria Anatolyivna, Professor, Department for Chemistry of Bioactive Nitrogen-Containing Heterocyclic Compounds, Institute of Bioorganic Chemistry and Petrochemistry of National Academy of Sciences of Ukraine, Ukraine.
(1) Bamidele Tajudeen, Nigerian Institute of Medical Research, Nigeria.
(2) Abhishek Kumar, Nitte University, India.
(3) Gokben Ozbey, Firat University, Vocational School of Health Services, Turkey.
Complete Peer review History: http://www.sciencedomain.org/review-history/22170




Antibiotics resistance genes (ARGs) in environmental samples have been implicated in the clinical spread of resistance to antibiotics. This study was therefore aimed at the metagenomic assessment of ARGs from various environmental samples. Benthic, epipellic, estuary and soil samples were collected and analyzed for physicochemical parameters using standard techniques and ARGs via metagenomics. Metagenomic DNA was extracted from the various samples and sequenced on Miseq Illumina platform. Following next generation sequencing, gene calling was performed on the assembled sequence reads using FragGeneScan to predict open reading frames (ORFs), which were functionally annotated to various taxonomic groups using the Kyoto Encyclopedia of Genes and Genomes (KEGG) and Ghost KOALA databases. Results of physicochemical analysis showed anthropogenic influence in the various environments. Furthermore, a total of 12 types of ARGs were identified, that belongs to the RND and ABC superfamilies of ARGs. These were dominated by five classes of bacteria which were Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, Deltaproteobacteria, and the Firmicutes (Clostridia). Our findings suggest that ARGs diversity differs with samples; with soil being the most abundant and only sample with the multidrug resistant efflux pumps. Further studies aimed at building a repository of ARGs genes in various environments should be carried out.


Keywords :

Antibiotics resistance genes; environment; efflux pumps; ABC transporters;   metagenomics.


Full Article - PDF    Page 1-10    Article Metrics

Review History    Comments

Search this site

Advanced Search

Announcement & News

Nature (Impact Factor: 41.6) confirmed high standard of SDI journal and its editors

We are happy to inform that Nature (Impact Factor: 41.6) confirmed high standard of SDI journal and ...

ISI Thomson Reuters selected British Journal of Pharmaceutical Research for Emerging Sources Citation Index

We are delighted to inform that ISI Thomson Reuters selected British Journal of Pharmaceutical Resea...

SCOPUS selected Annual Research & Review in Biology (ARRB)

We are delighted to inform that famous indexing organization SCOPUS (from Elsevier) selected  A...

Index Copernicus Evaluation Result Released

We are delighted to inform that Index Copernicus (a leading indexing organization from Pol...

Journal Repository (JR): Permanent Digital Archiving of SDI journals

SDI is happy to announce that all our journals are now permanently archived in Journal Repository (J...

SDI journal got 35th ranking in Publons

We are delighted to announce (as of 04/01/2016) that British Journal of Medicine and Medical Re...

Growth of SDI and world publication market

As of 2014, total 25,064 journals are competing in World market of journal publication. In 2011, tot...

Science (IF: 31) report confirmed the high standard of SDI journal

As per a recent report (Link) of Science journal (present Impact factor 31), one of our journal (Bri...

SDI introduced Post-publication peer review by its comment section

SDI journals encourage Post-publication peer review by its comment section   Policy details a...

SDI promotes transparent Advanced OPEN peer review

We have migrated to transparent and toughest ‘Advanced OPEN peer review’ system (...


  • No Awards listed.

Browser Compatibility : Mozila firefox, Google Crome and IE 7 & above. Creative Commons License Terms & Condition   |   Privacy Policy   |   Join Us   |   Help   |   Contact Us
© Copyright 2010-2018, SCIENCEDOMAIN international. All rights reserved.