Quick Menu

Upcoming Journals

Microbiology Research Journal International

Microbiology Research Journal International, 2456-7043,Vol.: 22, Issue.: 1

 

Original-research-article

 

Anti-Quorum Sensing Activity of Tetracera scandens and Aleurites moluccana Leaf Extracts against Chromobacterium violaceum

 

 

John Paul Matthew D. Guzman1,2,3* and Larry V. Padilla1

1College of Science, Pamantasan ng Lungsod ng Maynila, Intramuros, Manila, Philippines.

2The Graduate School, University of Santo Tomas, Sampaloc, Manila, Philippines.

3Environment and Biotechnology Division, Department of Science and Technology,  Industrial Technology Development Institute, Taguig City, Philippines.

 

Abstracts

 

Aims: To test the efficacy of Tetracera scandens (L.) Merr. and Aleurites moluccana (L.) Wiild. methanolic leaf extracts on the inhibition of quorum sensing-dependent pigmentation of Chromobacterium violaceum.

Study design:  In-vitro quorum sensing inhibition design was conducted.

Place and Duration of Study: The study was conducted at the Biology Laboratory, College of Science, Pamantasan ng Lungsod ng Maynila between November 2015 to May 2016.

Methodology: The leaf extracts were obtained through soaking in methanol and subsequent rotary evaporation. Qualitative screening of the anti-quorum sensing activities of the extracts were done through Agar Well Diffusion Assay. The Minimum Quorum Sensing Inhibition Concentration was determined through two-fold serial dilution and then wells were streaked on Mueller-Hinton Agar plates to identify the Minimum Bactericidal Concentration. Disc Diffusion Assay was done to quantitatively measure the anti-quorum sensing activities of the leaf extracts.

Results: The crude methanolic leaf extracts of T. scandens and A. moluccana were able to inhibit quorum-sensing on C. violaceum. The anti-QS activities of both plants were comparable at their Minimum Quorum Sensing Inhibitory Concentrations (P>.05), however, weaker activities were observed against the positive control (P = 0.001). The two extracts were partially active in inhibiting quorum sensing but were inactive in growth inhibition.

Conclusion: This study revealed that T. scandens and A. moluccana exhibit anti-quorum sensing activities against C. violaceum and these may be due to their phytochemical constituents, including flavonoids, terpenoids, and phenolics, which were previously proven to exhibit anti-quorum sensing activity. Extensive studies on the isolation of these compounds which may be responsible for their activities may be required to further improve the efficacy and harness the potential of these plants on quorum sensing inhibition.

 

Keywords :

Quorum sensing; quorum sensing inhibition; Tetracera scandens; malakatmon; Aleurites moluccana; lumbang; Chromobacterium violaceum.

 

Full Article - PDF    Page 1-10    Article Metrics

 

DOI : 10.9734/MRJI/2017/37450

Review History    Comments

Search this site

Advanced Search

Announcement & News

ISI Thomson Reuters selected British Journal of Pharmaceutical Research for Emerging Sources Citation Index

We are delighted to inform that ISI Thomson Reuters selected British Journal of Pharmaceutical Resea...

SCOPUS selected Annual Research & Review in Biology (ARRB)

We are delighted to inform that famous indexing organization SCOPUS (from Elsevier) selected  A...

Index Copernicus Evaluation Result Released

We are delighted to inform that Index Copernicus (a leading indexing organization from Pol...

Journal Repository (JR): Permanent Digital Archiving of SDI journals

SDI is happy to announce that all our journals are now permanently archived in Journal Repository (J...

Index Copernicus Value of British Journal of Medicine and Medical Research

  We are delighted to inform that Index Copernicus (a leading indexing organization ...

SDI journal got 35th ranking in Publons

We are delighted to announce (as of 04/01/2016) that British Journal of Medicine and Medical Re...

Growth of SDI and world publication market

As of 2014, total 25,064 journals are competing in World market of journal publication. In 2011, tot...

Publication and site statistics (up to Sept, 2015)

  Number of submission: 22181 (Total up to Sept, 2015) Number of published paper: 10949&n...

Index Medicus selected 15 SDI journals

We are delighted to inform that Index Medicus (IMSEAR) has selected following 15 SDI journals for in...

SCIENCEDOMAIN international received 5844 submissions in 2013

SCIENCEDOMAIN international is pleased to announce that it has received 5844 submissions in 2013, ac...



SCIENCEDOMAIN Awards

  • No Awards listed.

Browser Compatibility : Mozila firefox, Google Crome and IE 7 & above. Creative Commons License Terms & Condition   |   Privacy Policy   |   Join Us   |   Help   |   Contact Us
© Copyright 2010-2017, SCIENCEDOMAIN international. All rights reserved.