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Journal of Applied Life Sciences International, 2394-1103,Vol.: 14, Issue.: 2

Short Research Article

Effects of Ascorbic Acid, Dehydroascorbic Acid and Methotrexate on Breast Cancer Cell Viability


Yewande Dosunmu1 and Richard Owusu-Apenten1*

1Biomedical Sciences Research Institute, University of Ulster, Coleraine, BT52 1SA, United Kingdom.

Article Information
(1) Purnachandra Nagaraju Ganji, Department of Hematology and Medical Oncology, Emory University School of Medicine, USA.
(1) Jennifer Schroeder, Millikin University, USA.
(2) Viktoria Varvara Palla, Diak Klinikum Schwaebisch Hall, Germany.
Complete Peer review History: http://www.sciencedomain.org/review-history/21618


Aims: To examine the effects of ascorbic acid (AA), dehydroascorbic acid (DHA) and methotrexate (MTX) combined treatments on (MDA-MB-231) breast cancer cell viability and intracellular reactive oxygen species (ROS).

Study Design: In-vitro method.

Place and Duration of Study: Biomedical Sciences Research Institute, University of Ulster, Coleraine, BT52 1SA, United Kingdom. September 2016-2017

Methodology: Cytotoxicity tests were performed with MTX (0.01- 1000 µmol/l) alone or in combination with AA or DHA, for 72 h. Cell viability was measured by 3-4,5 dimethylthiazol-2,5 diphenyl tetrazolium bromide (MTT) or Sulforhodamine B (SRB) assays. Intracellular ROS was measured by 2’,7’-dichlorofluroscein diacetate assay.

Results: Treatments of MDA-MB231 cells with single agents, showed dose dependent response with 50% inhibition of cell viability (IC50) of 110.5-201.4 µmol/l (MTX), 2237-5703 µmol/l (AA) or 2474 µmol/l (DHA). Combination studies showed clear synergisms for MTX (~10 µmol/l) and DHA or AA (1100 µmol/l) but weak or no interactions at other concentrations. Three days combination treatment of DHA showed decrease of ROS, which was reversed by MTX (>10 µmol/l). 

Conclusions: Co-treatment of methotrexate with AA or DHA showed synergism (C1<1.0) and enhanced cytotoxicity of the anti-folate towards MDA-MB-231 breast cancer cells. Intracellular ROS decreased with AA and DHA treatment, which might be useful for reducing MTX-related oxidative stress.

Keywords :

Ascorbic acid; dehydroascorbic acid; methotrexate; breast cancer; MDA-MB-231 cells; Reactive oxygen species.

Full Article - PDF    Page 1-9

DOI : 10.9734/JALSI/2017/37185

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