+91 8617752708

International Journal of Biochemistry Research & Review, ISSN: 2231-086X,Vol.: 19, Issue.: 3

Original-research-article

Detection of Phytotoxin Produced from Leaf, Neck and Finger Blast Disease Causing Magnaporthe grisea through GC-MS Analysis

 

S. Shanmugapackiam1*, S. Parthasarathy1 and T. Raguchander1

1Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore – 641 003, India.

Article Information
Editor(s):
(1) Richard A. Manderville, Departments of Chemistry and Toxicology University of Guelph, Canada.
Reviewers:
(1) R. K. Dey, Central University of Jharkhand, India.
(2) A. Veera Reddy, India.
(3) F. Solano, University of Murcia, Campus Espinardo, Spain.
Complete Peer review History: http://www.sciencedomain.org/review-history/21514

Abstracts

Finger millet blast is the most devastating disease affecting different aerial parts of the plant at all growth stages starting from seedling to grain formation. Magnaporthe grisea being a ubiquitous pathogen with many hosts, understand the basis for host pathogen interaction and role of toxins will be useful in development of resistant varieties and their screening procedures. In this study, the presence of toxic volatile compounds were detected  by Thin Layer Chromatography (TLC) in the culture filtrate of leaf blast, neck blast and finger blast pathogen at different bands indicating various retention factor (Rf). Likewise the several toxic compounds were detected from M. grisea through GC-MS analysis from the semi-purified crude toxin in vitro viz., 1-Hexadecene (CAS), 1-Octadecene (CAS), Quinic acid,  1,2-Benzenedicarboxylic acid- bis (2-ethylhexyl) ester (CAS) from leaf blast isolate, Hippocasine, Holothurinogenin-2, Neophytadiene, 1,2-Benzene-dicarboxylic acid, diisooctyl ester (CAS), à-Patchoulene (CAS),  Synaptogenin B, 1-Naphthalenol, decahydro-1,4a-dimethyl-7-(1-methylethylidene),  Zingiberene (CAS) from neck blast isolate and 2H-Pyran-2-one, 6-hexyltetrahydro-delta-Hexylvalerolactone from finger blast isolate were detected.

Keywords :

Blast disease; GC-MS; Magnaporthe grisea; finger millet; toxic compounds.

Full Article - PDF    Page 1-10

DOI : 10.9734/IJBCRR/2017/33353

Review History    Comments

Our Contacts

Guest House Road, Street no - 1/6,
Hooghly, West Bengal,
India

+91 8617752708

 

Third Floor, 207 Regent Street
London, W1B 3HH,
UK

+44 20-3031-1429