International Journal of Biochemistry Research & Review, ISSN: 2231-086X,Vol.: 19, Issue.: 3
Detection of Phytotoxin Produced from Leaf, Neck and Finger Blast Disease Causing Magnaporthe grisea through GC-MS Analysis
S. Shanmugapackiam1*, S. Parthasarathy1 and T. Raguchander1 1Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore – 641 003, India.
S. Shanmugapackiam1*, S. Parthasarathy1 and T. Raguchander1
1Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore – 641 003, India.
(1) Richard A. Manderville, Departments of Chemistry and Toxicology University of Guelph, Canada.
(1) R. K. Dey, Central University of Jharkhand, India.
(2) A. Veera Reddy, India.
(3) F. Solano, University of Murcia, Campus Espinardo, Spain.
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Finger millet blast is the most devastating disease affecting different aerial parts of the plant at all growth stages starting from seedling to grain formation. Magnaporthe grisea being a ubiquitous pathogen with many hosts, understand the basis for host pathogen interaction and role of toxins will be useful in development of resistant varieties and their screening procedures. In this study, the presence of toxic volatile compounds were detected by Thin Layer Chromatography (TLC) in the culture filtrate of leaf blast, neck blast and finger blast pathogen at different bands indicating various retention factor (Rf). Likewise the several toxic compounds were detected from M. grisea through GC-MS analysis from the semi-purified crude toxin in vitro viz., 1-Hexadecene (CAS), 1-Octadecene (CAS), Quinic acid, 1,2-Benzenedicarboxylic acid- bis (2-ethylhexyl) ester (CAS) from leaf blast isolate, Hippocasine, Holothurinogenin-2, Neophytadiene, 1,2-Benzene-dicarboxylic acid, diisooctyl ester (CAS), à-Patchoulene (CAS), Synaptogenin B, 1-Naphthalenol, decahydro-1,4a-dimethyl-7-(1-methylethylidene), Zingiberene (CAS) from neck blast isolate and 2H-Pyran-2-one, 6-hexyltetrahydro-delta-Hexylvalerolactone from finger blast isolate were detected.
Blast disease; GC-MS; Magnaporthe grisea; finger millet; toxic compounds.
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