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International Journal of TROPICAL DISEASE & Health, ISSN: 2278-1005,Vol.: 26, Issue.: 4

Original-research-article

Isolation and Molecular Characterization of Dermatophytes in Donkeys

 

Elham Abdelbasit Suleiman1*, Wisal Gaafar Abdalla1, Ahmed Haroon Ahmed2 and Mohamed Awad Abdelgabar1

1Central Veterinary Research Laboratory, Department of Mycology, Animal Resources Research Corporation, P.O. Box 8067 (El Amarat), Khartoum, Sudan.

2Eldamazine Regional Veterinary Research Laboratory, Sudan.

Article Information
Editor(s):
(1) Ravindra Nath Sharma, Department of Pathobiology, School of Veterinary Medicine, St. George’s University, Grenada, West Indies.
Reviewers:
(1) Eliton da Silva Vasconcelos, Federal University of São Carlos, Brazil.
(2) Daisy Machado, Universidade Estadual de Campinas, Brazil.
Complete Peer review History: http://www.sciencedomain.org/review-history/21370

Abstracts

Background: Dermatophytosis is a fungal infection of skin, hair and nail caused by dermatophytes. The zoonotic nature of the disease requires early detection for implication of treatment.

Aim: The study was conducted to investigate into the cause of skin infection among donkeys in Eldamazine State, Central Sudan.

Study Design: This a case history studies over one year duration.

Methodology: A total of 31 samples of skin scrapings from donkeys with an age ranged 2-20years, from Eldamazie state, Sudan,were collected. Samples were cultured onto Sabouraud’S (SDA) media. The developed colonies were identified by convention method and characterized by molecular techniques using polymerase chain reaction.

Results: Trichophyton mentagrophytes and Trichophyton verrucosum were the predominant isolated dermatophytes revealed on investigation. Amplification of the β-tubulin gene, by polymerase chain reaction, and analysis of the amplicon sequence, further confirmed their identification.  The study revealed first report on characterization of these isolates by molecular methods using β- tubulin primers.

Conclusion: The present investigation showed that the integration of different methods and techniques led to identification of useful molecular marker for standardization of taxonomical studies of dermatophyte species.

Keywords :

Dermatophytes; βeta tubulin primers; conventional methods; PCR.

Full Article - PDF    Page 1-8

DOI : 10.9734/IJTDH/2017/36425

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